Graduate Program of Biomedical Sciences, University Center of Herminio Ometto Foundation - FHO, SP, Brazil.
Graduate Program of Cell Biology and Structural, University of Campinas, UNICAMP, SP, Brazil.
Biomed J. 2021 Dec;44(6):709-716. doi: 10.1016/j.bj.2020.05.014. Epub 2020 May 26.
The repair of burns in diabetic patients is a clinical problem. It is relevant to study alternative therapies that can improve the healing process. Our aim was to investigate the effects of Solidago chilensis associated or not with laser on burns in diabetic rats.
The animals were divided in four groups (n = 30): C- without treatment; S- S. chilensis extract; L-laser irradiated; LS- laser and S. chilensis. In 7, 14 and 21 days samples were collected after the injury to structural, morphometric and molecular analysis.
Our results demonstrate the association of S. chilensis and laser reduced the inflammatory infiltrate and favored the angiogenesis. In the groups treated only with laser or with the plant extract showed higher levels of VEGF. The low-level laser therapy (LLLT) promoted higher collagen I and reduction of collagen III. It was also observed higher MMP-2 activation and a decreasing of the active isoform of MMP-9 in the S, L and LS groups.
The treatments improved the repair of burns in diabetic rats, since it reduced the inflammatory infiltrate and favored the collagen organization presenting similar effects in the burn repair of the diabetics.
糖尿病患者的烧伤修复是一个临床问题。研究可以改善愈合过程的替代疗法具有重要意义。我们的目的是研究 Solidago chilensis 与激光联合或不联合治疗对糖尿病大鼠烧伤的影响。
将动物分为四组（n=30）：C-无治疗；S-Solidago chilensis 提取物；L-激光照射；LS-激光和 S. chilensis。在损伤后 7、14 和 21 天收集样品，进行结构、形态计量和分子分析。
我们的结果表明，Solidago chilensis 和激光的联合使用减少了炎症浸润，促进了血管生成。仅用激光或植物提取物治疗的组显示出更高水平的 VEGF。低水平激光疗法（LLLT）促进了更高水平的 I 型胶原蛋白和 III 型胶原蛋白的减少。还观察到 S、L 和 LS 组中 MMP-2 的激活增加和 MMP-9 的活性同工型减少。
这些治疗方法改善了糖尿病大鼠的烧伤修复，因为它减少了炎症浸润，促进了胶原组织的形成，在糖尿病患者的烧伤修复中表现出相似的效果。
