Taylor M B, Gutteridge W E
FEBS Lett. 1986 Apr 21;199(2):237-41. doi: 10.1016/0014-5793(86)80487-2.
LDH-X is the isoenzyme of lactate dehydrogenase found in mammalian spermatozoa, occurring in cytosolic and mitochondrial locations. Gossypol strongly inhibits it, and the spermicidal action of this compound is attributed to the disruption of a reducing shuttle. The flagellated protozoan, Trypanosoma cruzi, contains an enzyme activity similar to LDH-X, called alpha-hydroxy-acid dehydrogenase, which is here shown to possess cytosolic and glycosomal components. The glycosome is a microbody-like organelle containing the early glycolytic enzymes. We postulate that the inhibition of replication of T. cruzi by gossypol derives from interference with glycosomal reducing shuttles. T. lewisi resembles T. cruzi in this respect.
乳酸脱氢酶X（LDH-X）是在哺乳动物精子中发现的乳酸脱氢酶同工酶，存在于细胞质和线粒体中。棉酚能强烈抑制它，该化合物的杀精作用归因于还原穿梭的破坏。有鞭毛的原生动物克氏锥虫含有一种类似于LDH-X的酶活性，称为α-羟基酸脱氢酶，本文显示它具有细胞质和糖体成分。糖体是一种类似微体的细胞器，含有早期糖酵解酶。我们推测棉酚对克氏锥虫复制的抑制源于对糖体还原穿梭的干扰。在这方面，路易斯锥虫与克氏锥虫相似。
