Gerez de Burgos N M, Burgos C, Montamat E E, Rovai L E, Blanco A
Biochem Pharmacol. 1984 Apr 1;33(7):955-9. doi: 10.1016/0006-2952(84)90500-8.
The effects of gossypol, a polyphenolic compound isolated from the cotton plant upon six oxidoreductases from cultured epimastigotes of Typanosoma cruzi were studied. Gossypol was a powerful inhibitor of the alpha-hydroxyacid and malate dehydrogenases, NAD-linked enzymes, and of glutamate dehydrogenase, malic enzyme and glucose-6-phosphate dehydrogenase, NADP-dependent enzymes. The drug did not have an effect on succinate dehydrogenase, a flavoprotein. The Ki values with respect to substrate were 0.73, 0.3 and 3.5 microM for alpha-hydroxyacid, malate and glutamate dehydrogenases, respectively, and 1.1, 0.19 and 7.8 microM with respect to the coenzyme. Inhibition was noncompetitive with respect to substrate and uncompetitive in relation to the coenzyme.
研究了从棉花植物中分离出的多酚化合物棉酚对克氏锥虫培养型上鞭毛体的六种氧化还原酶的影响。棉酚是α-羟酸脱氢酶、苹果酸脱氢酶（NAD连接酶）、谷氨酸脱氢酶、苹果酸酶和葡萄糖-6-磷酸脱氢酶（NADP依赖酶）的强力抑制剂。该药物对黄素蛋白琥珀酸脱氢酶没有作用。相对于底物，α-羟酸脱氢酶、苹果酸脱氢酶和谷氨酸脱氢酶的Ki值分别为0.73、0.3和3.5微摩尔，相对于辅酶的Ki值分别为1.1、0.19和7.8微摩尔。抑制作用相对于底物是非竞争性的，相对于辅酶是反竞争性的。
