Department of genetics and cell biology, Basic medical college, Qingdao University, Qingdao, China, 266071.
Department of Reproduction, The Affiliated Hospital of Qingdao University, Qingdao, China, 266000.
Food Funct. 2022 Mar 7;13(5):2631-2646. doi: 10.1039/d1fo03741b.
C-phycocyanin from has pharmacological effects such as anti-oxidation, anti-cancer, anti-inflammatory and anti-atherosclerosis activities as well as liver and kidney protection. However, there is little research on C-phycocyanin applied in the field of reproductive medicine, and it is therefore the focus of the current study. In this study, a GC-1 spg cell model and male mouse reproductive injury model were constructed by TNF α + Smac mimetic + zVAD-fmk (TSZ) and cyclophosphamide (Cy), respectively. It has been proved that C-phycocyanin can increase cell viability and reduce cell death in GC-1 spg cells induced by TSZ. C-phycocyanin could protect the reproductive system of male mice from cyclophosphamide, improve spermatogenesis, sperm quality and fertility, increase the release of testosterone, stabilize the feedback regulation mechanism, and ensure the spermatogenic ability of mice. It could also improve the ability of anti-oxidation. In addition, C-phycocyanin could play a protective role by down-regulating RIPK1, RIPK3, and p-MLKL to inhibit the necroptotic signaling pathway. These results suggest that C-phycocyanin could protect GC-1 spg cells and the reproductive system of male mice from TSZ and cyclophosphamide, and the protective mechanism may be achieved by inhibiting the signal pathway of necroptosis. Therefore, C-phycocyanin could serve as a promising reproductive system protective agent. C-phycocyanin may enter public life as a health product in the future.
藻蓝蛋白具有抗氧化、抗癌、抗炎、抗动脉粥样硬化以及保护肝脏和肾脏等药理作用。然而，关于藻蓝蛋白在生殖医学领域的应用研究较少，因此是当前研究的重点。本研究通过 TNFα+Smac 模拟物+zVAD-fmk（TSZ）和环磷酰胺（Cy）分别构建 GC-1 spg 细胞模型和雄性小鼠生殖损伤模型。证明藻蓝蛋白可增加 TSZ 诱导的 GC-1 spg 细胞活力，减少细胞死亡。藻蓝蛋白可保护雄性小鼠生殖系统免受环磷酰胺的影响，改善精子发生、精子质量和生育能力，增加睾酮的释放，稳定反馈调节机制，确保小鼠的生精能力。还可以提高抗氧化能力。此外，藻蓝蛋白通过下调 RIPK1、RIPK3 和 p-MLKL 抑制坏死性信号通路发挥保护作用。这些结果表明，藻蓝蛋白可保护 GC-1 spg 细胞和雄性小鼠的生殖系统免受 TSZ 和环磷酰胺的损伤，其保护机制可能是通过抑制坏死性信号通路实现的。因此，藻蓝蛋白可作为一种有前途的生殖系统保护剂。藻蓝蛋白未来可能作为保健品进入公众生活。
