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通过基因工程过表达卟啉途径基因促进 sp. PCC 6803 中血红素和藻蓝蛋白的生物合成。

Promoting Heme and Phycocyanin Biosynthesis in sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering.

机构信息

School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, China.

School of Pharmacy, Binzhou Medical University, Yantai 264003, China.

出版信息

Mar Drugs. 2023 Jul 13;21(7):403. doi: 10.3390/md21070403.

DOI:10.3390/md21070403
PMID:37504934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10382063/
Abstract

Due to their unique biochemical and spectroscopic properties, both heme and phycocyanobilin are widely applied in the medical and food industries. sp. PCC 6803 contains both heme and phycocyanin, and is capable of synthesizing phycocyanin using heme as a precursor. The aim of this study was to uncover viable metabolic targets in the porphyrin pathway from sp. PCC 6803 to promote the accumulation of heme and phycocyanin in the recombinant strains of microalgae. A total of 10 genes related to heme synthesis pathway derived from PCC 7942 and 12 genes related to endogenous heme synthesis were individually overexpressed in strain PCC 6803. The growth rate and pigment content (heme, phycocyanin, chlorophyll a and carotenoids) of 22 recombinant algal strains were characterized. Quantitative real-time PCR technology was used to investigate the molecular mechanisms underlying the changes in physiological indicators in the recombinant algal strains. Among the 22 mutant strains, the mutant overexpressing the haemoglobin gene () of strain PCC 6803 had the highest heme content, which was 2.5 times higher than the wild type; the mutant overexpressing the gene of strain PCC 7942 () had the highest phycocyanin content, which was 4.57 times higher than the wild type. Overall, the results suggest that genes in the porphyrin pathway could significantly affect the heme and phycocyanin content in strain PCC 6803. Our study provides novel crucial targets for promoting the accumulation of heme and phycocyanin in cyanobacteria.

摘要

由于血红素和藻蓝胆素具有独特的生化和光谱特性，因此它们被广泛应用于医学和食品工业。 sp. PCC 6803 同时含有血红素和藻蓝蛋白，并且能够使用血红素作为前体合成藻蓝蛋白。本研究旨在揭示 sp. PCC 6803 中卟啉途径中的可行代谢靶标，以促进重组微藻中血红素和藻蓝蛋白的积累。从 PCC 7942 中获得了与血红素合成途径相关的 10 个基因和与内源性血红素合成相关的 12 个基因，分别在 PCC 6803 中过表达。对 22 株重组藻株的生长速率和色素含量（血红素、藻蓝蛋白、叶绿素 a 和类胡萝卜素）进行了表征。利用定量实时 PCR 技术研究了重组藻株生理指标变化的分子机制。在 22 株突变株中，过表达 PCC 6803 菌株血红蛋白基因 () 的突变株血红素含量最高，是野生型的 2.5 倍；过表达 PCC 7942 菌株基因 () 的突变株藻蓝蛋白含量最高，是野生型的 4.57 倍。总体而言，结果表明卟啉途径中的基因可显著影响 PCC 6803 中的血红素和藻蓝蛋白含量。本研究为促进蓝藻中血红素和藻蓝蛋白的积累提供了新的关键靶标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/693d7635e1ff/marinedrugs-21-00403-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/6cf4bacb85c4/marinedrugs-21-00403-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/ca4a25e1f037/marinedrugs-21-00403-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/de6b1eb79857/marinedrugs-21-00403-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/91ef4f295476/marinedrugs-21-00403-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/d19f2f19ef95/marinedrugs-21-00403-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/c458fcd345b2/marinedrugs-21-00403-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/271f5836f938/marinedrugs-21-00403-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/7c49860dac7a/marinedrugs-21-00403-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/693d7635e1ff/marinedrugs-21-00403-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/6cf4bacb85c4/marinedrugs-21-00403-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/ca4a25e1f037/marinedrugs-21-00403-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/de6b1eb79857/marinedrugs-21-00403-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/91ef4f295476/marinedrugs-21-00403-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/d19f2f19ef95/marinedrugs-21-00403-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/c458fcd345b2/marinedrugs-21-00403-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/271f5836f938/marinedrugs-21-00403-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/7c49860dac7a/marinedrugs-21-00403-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ae4/10382063/693d7635e1ff/marinedrugs-21-00403-g009.jpg

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