Department of Psychology, University of Alabama at Birmingham, United States.
Department of Psychology, University of Alabama at Birmingham, United States.
Physiol Behav. 2022 May 1;248:113740. doi: 10.1016/j.physbeh.2022.113740. Epub 2022 Feb 12.
Type 2 diabetes (T2D) costs billions of dollars annually, is also associated with pain (diabetic neuropathy), as well as retinopathy, lower urinary tract/urinary bladder dysfunction, depression, and systemic inflammation, affecting quality of life for patients. To that end, animal models are utilized to explore potential treatments, but may not reflect the complexity of the condition.
We aimed to test an improved model of T2D that more closely mimics the clinical mechanisms and symptoms in an outbred strain of mouse.
Male and female CD-1 mice (n = 72) were fed one of four diets: regular chow (REG), our Standard American Diet (SAD), a revised SAD (SAD2), or the commonly-used high-fat diet (HFD). Overall, HFD- and SAD-fed mice had significant weight gain and increased fat mass. Following injury, the SAD- and SAD2-fed mice showed protracted recovery, but the HFD-fed mice did not. Similarly, SAD- and SAD2-fed mice showed impaired retinal function compared to REG-fed mice, but the HFD-fed mice did not.
The SAD and SAD2 more closely model the problematic dietary intake and subsequent clinical symptoms associated with T2D.
The adjusted SAD2 may be a better representation of a human-translatable diet than the SAD and HFD, and may allow for increased advances in the investigation of T2D-related symptoms.
2 型糖尿病（T2D）每年耗费数十亿美元，还与疼痛（糖尿病性神经病变）以及视网膜病变、下尿路/膀胱功能障碍、抑郁和全身炎症相关，影响患者的生活质量。为此，人们利用动物模型来探索潜在的治疗方法，但这些模型可能无法反映疾病的复杂性。
我们旨在测试一种改进的 T2D 模型，该模型更能模拟出近交系小鼠的临床机制和症状。
雄性和雌性 CD-1 小鼠（n=72）分别喂食以下四种饮食中的一种：常规饮食（REG）、我们的标准美国饮食（SAD）、修订后的 SAD（SAD2）或常用的高脂肪饮食（HFD）。总体而言，HFD 和 SAD 饮食的小鼠体重明显增加，体脂增加。受伤后，SAD 和 SAD2 饮食的小鼠恢复时间延长，但 HFD 饮食的小鼠没有。同样，与 REG 饮食的小鼠相比，SAD 和 SAD2 饮食的小鼠视网膜功能受损，但 HFD 饮食的小鼠没有。
SAD 和 SAD2 更能模拟与 T2D 相关的有问题的饮食摄入和随后的临床症状。
调整后的 SAD2 可能比 SAD 和 HFD 更能代表可转化为人类的饮食，并且可能会促进对 T2D 相关症状的研究进展。
