Fadwa El-Ouady, Amthaghri Smail, Akdad Mourad, El-Haidani Ahmed, Eddouks Mohamed
Team of Ethnopharmacology and Pharmacognosy, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, 52000. Errachidia. Morocco.
Cardiovasc Hematol Disord Drug Targets. 2022 Feb 16. doi: 10.2174/1871529X22666220216102243.
The study aimed to assess the antihyperglycemic activity of Pelargonium graveolens.
Pelargonium graveolens (P. graveolens) (Geraniaceae) is a medicinal plant frequently used in traditional medicine in Morocco and Algeria as remedy against a multitude of illnesses. Interestingly, it is used in the Moroccan pharmacopeia for managing diabetes.
The current study was carried out in order to assess the antihyperglycemic activity of P. graveolens in normal and streptozotocin(STZ)-induced diabetic rats, as well as to evaluate the acute toxicity of the leaves aqueous extract of Pelargonium graveolens (PGLAE).
The therapeutic effect of PGLAE at a dose of 40 mg/kg on blood glucose levels was investigated in normal and STZ-induced diabetic rats after a single oral (6h) and a repeated (15 days) oral administration. The acute toxicity of the PGLAE was examined in accordance the OECD test guideline. Post-treatment, body weight, signs of toxicity, and/or mortality were observed for 14 days. Further assays such as the oral glucose tolerance test, histopathological examination and antioxidant activity was also performed according to standard protocols.
Findings exhibited that both single and repeated oral doses of PGLAE (40 mg/kg) produced significant reduction of blood glucose levels in normal and diabetic rats. All treated animals survived and no apparent adverse effects were observed during the duration of the acute toxicity study. Importantly, oral LD50 of PGLAE was greater than 2000 mg/kg BW. Furthermore, a remarkable influence on glucose tolerance was also noticed after PGLAE treatment. Moreover, PGLAE was able to improve histopathological structure of liver and possesses a potential antioxidant effect in vitro.
In conclusion, this study reports the antidiabetic effect of PGLAE in rats supporting then its traditional use for the management of diabetes.
本研究旨在评估香叶天竺葵的降血糖活性。
香叶天竺葵（牻牛儿苗科）是一种药用植物，在摩洛哥和阿尔及利亚的传统医学中常用于治疗多种疾病。有趣的是，它被收录在摩洛哥药典中用于治疗糖尿病。
开展本研究以评估香叶天竺葵在正常和链脲佐菌素（STZ）诱导的糖尿病大鼠中的降血糖活性，并评估香叶天竺葵叶水提取物（PGLAE）的急性毒性。
在正常和STZ诱导的糖尿病大鼠中，单次口服（6小时）和重复口服（15天）40mg/kg剂量的PGLAE后，研究其对血糖水平的治疗效果。根据经合组织测试指南检测PGLAE的急性毒性。治疗后，观察14天的体重、毒性体征和/或死亡率。还根据标准方案进行了口服葡萄糖耐量试验、组织病理学检查和抗氧化活性等进一步检测。
研究结果表明，单次和重复口服PGLAE（40mg/kg）均可使正常和糖尿病大鼠的血糖水平显著降低。所有受试动物均存活，在急性毒性研究期间未观察到明显的不良反应。重要的是，PGLAE的口服半数致死量大于2000mg/kg体重。此外，PGLAE治疗后对葡萄糖耐量也有显著影响。此外，PGLAE能够改善肝脏的组织病理学结构，并在体外具有潜在的抗氧化作用。
总之，本研究报告了PGLAE在大鼠中的抗糖尿病作用，支持了其在糖尿病治疗中的传统用途。
