Nguyen Hung Van, Nguyen Ha Thu Thi, Le Nhan Trong, Tran Trang Quynh, Nguyen Loan Thanh Thi, Mai Thanh Phuong, Pham Phong Xuan, Pham Anh Van Thi, Nguyen Hoai Thi
Faculty of Traditional Medicine, Hanoi Medical University, Hanoi, Vietnam.
Faculty of Traditional Medicine, Hue University of Medicine and Pharmacy, Hue University, Hue City, Vietnam.
Basic Clin Pharmacol Toxicol. 2025 Aug;137(2):e70081. doi: 10.1111/bcpt.70081.
The acute and subchronic toxicity, along with the anti-type 2 diabetic effects, of a triterpenoid extract from persimmon leaves (Tri DKL) was evaluated in animals. Acute oral toxicity was assessed in Swiss mice, whereas subchronic toxicity was investigated in Wistar rats given Tri DKL at 125 and 375 mg/kg body weight (BW) daily for 90 days. Type 2 diabetes was induced in Swiss mice via an 8-week high-fat diet, followed by a single intraperitoneal injection of streptozotocin (100 mg/kg BW). Diabetic mice were subsequently treated with Tri DKL at 250 and 750 mg/kg BW/day for 2 weeks. Results showed that Tri DKL, even at the highest dose of 2500 mg/kg, did not produce any signs of acute toxicity in mice. In rats, subchronic administration of 125 and 375 mg/kg BW/day caused no significant alterations in general behaviours, haematological parameters or hepatic/renal function markers. In diabetic mice, Tri DKL significantly reduced blood glucose levels at both doses. It also lowered total cholesterol and hepatic malondialdehyde levels. Notably, at 250 mg/kg BW/day, Tri DKL decreased triglyceride levels while improving liver and pancreatic tissue histology. Overall, Tri DKL exhibited no acute or subchronic toxicity in animals and demonstrated hypoglycemic and lipid-lowering effects in type 2 diabetic mice, suggesting potential therapeutic benefits.
对柿子叶三萜提取物（Tri DKL）的急性和亚慢性毒性以及抗2型糖尿病作用进行了动物实验评估。在瑞士小鼠中评估急性经口毒性，而在体重125和375mg/kg的Wistar大鼠中研究亚慢性毒性，连续90天每日给予Tri DKL。通过8周高脂饮食诱导瑞士小鼠患2型糖尿病，随后单次腹腔注射链脲佐菌素（100mg/kg体重）。随后，糖尿病小鼠以250和750mg/kg体重/天的剂量接受Tri DKL治疗2周。结果表明，即使在2500mg/kg的最高剂量下，Tri DKL在小鼠中也未产生任何急性毒性迹象。在大鼠中，每天125和375mg/kg体重的亚慢性给药对一般行为、血液学参数或肝/肾功能指标均无显著影响。在糖尿病小鼠中，两种剂量的Tri DKL均显著降低血糖水平。它还降低了总胆固醇和肝脏丙二醛水平。值得注意的是，在250mg/kg体重/天时，Tri DKL降低了甘油三酯水平，同时改善了肝脏和胰腺组织的组织学。总体而言，Tri DKL在动物中未表现出急性或亚慢性毒性，并在2型糖尿病小鼠中显示出降血糖和降血脂作用，提示其具有潜在的治疗益处。
