Reimer L G, Kepas A
J Clin Microbiol. 1986 Feb;23(2):377-8. doi: 10.1128/jcm.23.2.377-378.1986.
Although the Gram stain is usually used to screen sputum specimens prior to culture, wet mount observation with Nomarski optics has been suggested as a useful alternative. We compared the two methods and found that more specimens were rejected by the Gram stain technique without eliminating any that yielded important clinical information.
虽然革兰氏染色通常用于在培养前筛查痰标本，但有人建议使用诺马斯基光学显微镜进行湿片观察作为一种有用的替代方法。我们比较了这两种方法，发现革兰氏染色技术拒收的标本更多，却并未排除任何能提供重要临床信息的标本。
