Blyden G T, Franklin C, Cho S I, Kaplan M M, Hirsch C A, Greenblatt D J
J Clin Pharmacol. 1986 May-Jun;26(5):367-71. doi: 10.1002/j.1552-4604.1986.tb03539.x.
Cyclosporine blood concentrations were simultaneously determined by radioimmunoassay (RIA) and high-performance liquid chromatography (HPLC) at multiple points in time in two patients receiving cyclosporine for immunosuppression following liver transplantation. Radioimmunoassay levels always exceed those determined by HPLC; however, the divergence between the two methods increased as serum bilirubin concentration increased, with HPLC:RIA ratios generally less than 0.3 when serum bilirubin concentrations exceeded 10.0 mg/dL. These preliminary results suggest that retention of immunoactive cyclosporine metabolites due to imparied liver function may account for RIA-determined cyclosporine concentrations that greatly exceed those measured by HPLC.
在两名肝移植后接受环孢素免疫抑制治疗的患者中，通过放射免疫分析（RIA）和高效液相色谱法（HPLC）在多个时间点同时测定环孢素血药浓度。放射免疫分析测定的水平总是超过高效液相色谱法测定的水平；然而，随着血清胆红素浓度升高，两种方法之间的差异增大，当血清胆红素浓度超过10.0mg/dL时，高效液相色谱法与放射免疫分析法的比值通常小于0.3。这些初步结果表明，由于肝功能受损导致免疫活性环孢素代谢物潴留，可能是放射免疫分析测定的环孢素浓度大大超过高效液相色谱法测定值的原因。
