Said J W, Sassoon A F, Shintaku I P, Chien K, Pinkus G S
J Histochem Cytochem. 1986 May;34(5):607-11. doi: 10.1177/34.5.3517145.
Monoclonal antibodies B1 and B2 are thought to recognize B-lineage restricted antigens, and have been used to define stages of B-cell maturation and characterize B-cell lymphomas. Immunostaining on cryostat sections has revealed a puzzling dendritic or extracellular pattern of staining for B2 within germinal centers and neoplastic follicles. In this study B1 and B2 are localized precisely on hyperplastic and neoplastic lymphoid tissues using immuno-ultrastructural techniques on cryostat sections, cell suspensions, and cell monolayers. B1 and B2 were localized to cell surfaces, including microvillous surface projections, on small and large transformed normal and neoplastic B lymphocytes. B2, in addition to staining in lymphoid cells, was localized to anastomosing cytoplasmic processes of dendritic histiocytes. These findings explain the apparently extracellular localization of B2 in cryostat sections and indicate that patterns of staining for B2 may represent a combination of staining on lymphoid cells and dendritic histiocytes.
单克隆抗体B1和B2被认为可识别B系限制性抗原，并已用于确定B细胞成熟阶段及表征B细胞淋巴瘤。对低温恒温器切片进行免疫染色显示，生发中心和肿瘤性滤泡内的B2呈现出令人费解的树突状或细胞外染色模式。在本研究中，利用低温恒温器切片、细胞悬液和细胞单层上的免疫超微结构技术，将B1和B2精确地定位在增生性和肿瘤性淋巴组织上。B1和B2定位于正常和肿瘤性B淋巴细胞大小转变型细胞的细胞表面，包括微绒毛表面突起。除了在淋巴细胞中染色外，B2还定位于树突状组织细胞吻合的细胞质突起上。这些发现解释了低温恒温器切片中B2明显的细胞外定位，并表明B2的染色模式可能代表淋巴细胞和树突状组织细胞染色的组合。
