Tabibzadeh S S, Gerber M A
J Histochem Cytochem. 1985 Sep;33(9):884-90. doi: 10.1177/33.9.2410482.
In order to evaluate the applicability of imprints to immuno-ultrastructural studies of lymphoid tissues, we compared distribution pattern and morphology of B cells, T cells, T-cell subsets, and follicular dendritic reticulum cells (DRC) at the light and ultrastructural level in imprints and sections of tonsils and lymph nodes. The surface antigenic profile of lymphoid cells was revealed with monoclonal antibodies in an avidin-biotin-peroxidase complex (ABC) method. Distribution of lymphoid cells in coherent areas of imprints recapitulated their disposition in sections of respective lymphoid tissues. Preservation of microanatomical relationships and ultrastructure of lymphoid cells in imprints allowed evaluation of associations and fine structural detail of lymphoid cells. Morphologic configurations of B cells in imprints, confined to round aggregates, were similar to fine structural morphology of B cells in mantle zones (MZ) and germinal centers (GC). Processes of DRCs in imprints formed conformations resembling their meshwork within follicles and mantled lymphoid cells. In imprints and sections, lymphocytes of cytotoxic/suppressor phenotype had a large amount of cytoplasm with many organelles. In contrast, cells of helper/inducer phenotype displayed a high nucleocytoplasmic (N/C) ratio and small numbers of organelles. Thus, imprints represent an easy, fast, and reliable method that lends itself to immunoultrastructural studies of lymphoid tissues.
为了评估印片在淋巴组织免疫超微结构研究中的适用性，我们在扁桃体和淋巴结的印片及切片中，在光镜和超微结构水平上比较了B细胞、T细胞、T细胞亚群和滤泡树突状网状细胞（DRC）的分布模式和形态。采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，用单克隆抗体揭示淋巴细胞的表面抗原谱。印片中连续区域内淋巴细胞的分布反映了它们在相应淋巴组织切片中的分布情况。印片中淋巴细胞微解剖关系和超微结构的保存，使得对淋巴细胞的关联和精细结构细节进行评估成为可能。印片中局限于圆形聚集物的B细胞形态结构，类似于套区（MZ）和生发中心（GC）中B细胞的精细结构形态。印片中DRC的突起形成的构象类似于它们在滤泡和被膜淋巴细胞内的网络结构。在印片和切片中，细胞毒性/抑制性表型的淋巴细胞有大量含有许多细胞器的细胞质。相比之下，辅助/诱导性表型的细胞表现出高核质（N/C）比和少量细胞器。因此，印片是一种简便、快速且可靠的方法，适用于淋巴组织的免疫超微结构研究。
