Kurki P, Helve T, Dahl D, Virtanen I
J Rheumatol. 1986 Feb;13(1):69-73.
Autoantibodies against neuronal antigens occur in sera of patients with systemic lupus erythematosus (SLE). These antibodies may have significance in the pathogenesis of neurological complications of SLE. However, the neuronal structures containing the corresponding autoantigens are poorly known. In our study we assayed circulating antibodies against defined neuronal components--neurofilaments--by an enzyme-linked immunosorbent assay (ELISA) using purified neurofilament polypeptides as targets. Circulating neurofilament antibodies (anti-NF) of IgG class were detected in 21% of 28 patients with SLE and in 6% of 17 patients with rheumatoid arthritis and in none of the 14 patients with primary sicca syndrome and 40 blood donors. The presence of anti-NF could also be confirmed by the indirect immunofluorescence technique using frozen sections of rat spinal cord. In one serum, anti-NF cross reacted with vimentin type of intermediate filaments. The antibodies bound both to the 70 kilodalton and the 200 kilodalton polypeptides of neurofilaments as judged by the immunoblotting technique. Two of 6 anti-NF positive patients had neurological complications.
系统性红斑狼疮（SLE）患者血清中存在针对神经元抗原的自身抗体。这些抗体可能在SLE神经并发症的发病机制中具有重要意义。然而，含有相应自身抗原的神经元结构却鲜为人知。在我们的研究中，我们使用纯化的神经丝多肽作为靶点，通过酶联免疫吸附测定（ELISA）来检测针对特定神经元成分——神经丝的循环抗体。在28例SLE患者中，21%检测到IgG类循环神经丝抗体（抗NF）；在17例类风湿性关节炎患者中，6%检测到该抗体；而在14例原发性干燥综合征患者和40名献血者中均未检测到。使用大鼠脊髓冰冻切片的间接免疫荧光技术也证实了抗NF的存在。在一份血清中，抗NF与波形蛋白类型的中间丝发生交叉反应。通过免疫印迹技术判断，这些抗体与神经丝的70千道尔顿和200千道尔顿多肽均结合。6例抗NF阳性患者中有2例出现神经并发症。
