Kurki P, Gripenberg M, Partanen P, Helve T
Department of Pathology, University of Helsinki, Finland.
J Clin Pathol. 1987 Dec;40(12):1475-80. doi: 10.1136/jcp.40.12.1475.
Three hundred and one sera from patients with rheumatic and other diseases were investigated using a simple enzyme immunoassay for screening of rheumatoid factors and antinuclear antibodies. The assay had a sensitivity of 77% for systemic lupus erythematosus, 90% for the primary sicca syndrome, and 89% for rheumatoid arthritis. Only 13% of sera from patients with chronic non-rheumatic diseases were positive. The test was further evaluated in a group of patients with suspected rheumatic disease who were followed up for six to 12 months. The test was positive in 16 of 17 sera from patients with connective tissue diseases but in only seven of 36 sera (19%) from patients with non-inflammatory joint diseases. None of the four patients with reactive arthritis was positive by this test. The sensitivity of the assay was comparable with that of the agglutination and immunofluorescence tests for rheumatoid factors and antinuclear factors. For the screening of rheumatoid factor and antinuclear antibodies this kind of test panel offers a simple alternative to the conventional tests for small clinical laboratories and for those in which the autoantibody tests could be automated, as the assay can be performed in one working day and only one dilution of serum is needed to obtain a quantitative result.
采用一种简单的酶免疫测定法对301份风湿性疾病及其他疾病患者的血清进行检测，以筛查类风湿因子和抗核抗体。该测定法对系统性红斑狼疮的敏感度为77%，对原发性干燥综合征为90%，对类风湿关节炎为89%。慢性非风湿性疾病患者的血清仅有13%呈阳性。在一组疑似风湿性疾病的患者中对该检测进行了进一步评估，这些患者接受了6至12个月的随访。结缔组织病患者的17份血清中有16份检测呈阳性，但非炎性关节病患者的36份血清中只有7份（19%）呈阳性。反应性关节炎患者的4例中无一例通过该检测呈阳性。该测定法的敏感度与类风湿因子和抗核因子的凝集试验及免疫荧光试验相当。对于类风湿因子和抗核抗体的筛查，这种检测方法为小型临床实验室以及那些能够实现自身抗体检测自动化的实验室提供了一种简单的替代传统检测的方法，因为该测定法可在一个工作日内完成，且仅需对血清进行一次稀释即可获得定量结果。
