King R J, Cano A, Finley J, Coffer A I
J Steroid Biochem. 1986 Jan;24(1):369-72. doi: 10.1016/0022-4731(86)90084-1.
Monoclonal antibodies have been prepared against a soluble oestradiol receptor (REC) preparation partially purified from human myometrium by oestradiol affinity chromatography. The antibodies were detected by their ability to immunoprecipitate receptor bound [125I] oestradiol. One of the antibodies (D5) has been studied in detail. It will only precipitate REC after activation by salt, heat, low pH or KCNS and will not react with nuclear RE. It will not react with androgen, progesterone or glucocorticoid receptors nor with sex hormone binding globulin; it will only combine with REC from human sources. D5 recognizes a cytoplasmic 29 kdalton protein (p29) that can be separated from both type I and II soluble oestradiol binding proteins. p29 can react with activated REC and is qualitatively and quantitatively related to REC. IRMA and histochemical methods have been developed for quantitating p29 and relating its amount to receptors in human breast tumours. With both methods, highly significant (P less than 0.001) correlations with REC but not RP have been obtained. Both methods indicate that many REC-RP+ tumours contain p29. The histochemical method detects marked cellular heterogeneity in some tumours. The function of p29 is not known. It is an REC-related antigen that may be a previously undetected component of the oestradiol receptor machinery.
已制备出针对通过雌二醇亲和层析从人子宫肌层部分纯化得到的可溶性雌二醇受体（REC）制剂的单克隆抗体。通过其免疫沉淀与受体结合的[125I]雌二醇的能力来检测这些抗体。其中一种抗体（D5）已得到详细研究。它仅在经盐、热、低pH或硫氰酸钾激活后才会沉淀REC，且不会与核受体反应。它不会与雄激素、孕激素或糖皮质激素受体反应，也不会与性激素结合球蛋白反应；它仅与人源的REC结合。D5识别一种细胞质29千道尔顿蛋白（p29），该蛋白可与I型和II型可溶性雌二醇结合蛋白分离。p29可与活化的REC反应，且在质量和数量上与REC相关。已开发出免疫放射测定法（IRMA）和组织化学方法来定量p29，并将其含量与人类乳腺肿瘤中的受体相关联。使用这两种方法，均获得了与REC高度显著（P小于0.001）的相关性，但与核受体（RP）无相关性。两种方法均表明，许多REC-RP阳性肿瘤含有p29。组织化学方法检测到一些肿瘤中存在明显的细胞异质性。p29的功能尚不清楚。它是一种与REC相关的抗原，可能是雌二醇受体机制中先前未被检测到的成分。
