Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Xuzhou, Jiangsu 221131, China.
J Agric Food Chem. 2022 Mar 2;70(8):2574-2588. doi: 10.1021/acs.jafc.1c05388. Epub 2022 Feb 17.
Sweetpotato [ (L.) Lam.] is a major tuberous root crop that is rich in flavonoids. Here, we discovered a spontaneous mutation in the color of the leaf vein base (LVB) and root skin (RS) in the Zheshu 81 cultivar. The flavonoid and anthocyanin metabolites and molecular mechanism were analyzed using metabolome and transcriptome data. Compared to the wild type, 13 differentially accumulated metabolites (DAMs) in the LVB and 59 DAMs in the RS were all significantly downregulated. Moreover, all the anthocyanin metabolites decreased significantly. The differentially expressed genes (DEGs) encoding the key enzymes in the later enzymatic reaction of anthocyanin and flavonoid were significantly downregulated in the mutant. The expression trends of the transcription factor MYB were evidently related to the anthocyanin content. These results offer insights into the coloration in the LVB and RS and a theoretical basis for determining the regulation of flavonoid and anthocyanin synthesis in sweetpotato.
甘薯 [(L.) Lam.] 是一种富含类黄酮的主要块根作物。在这里，我们在浙薯 81 品种中发现了叶片脉络基部 (LVB) 和根皮 (RS) 颜色的自发突变。利用代谢组学和转录组学数据对类黄酮和花青素代谢物和分子机制进行了分析。与野生型相比，LVB 中有 13 种差异积累代谢物 (DAMs) 和 RS 中有 59 种 DAMs 均显著下调。此外，所有的花青素代谢物均显著下降。突变体中花青素和类黄酮后期酶反应关键酶编码的差异表达基因 (DEGs) 显著下调。转录因子 MYB 的表达趋势与花青素含量明显相关。这些结果为 LVB 和 RS 的着色提供了深入了解，并为确定甘薯中类黄酮和花青素合成的调控提供了理论依据。
