三种不同类型癌症中 microRNA 表达定量分析的参考基因选择。
Selection of reference genes for quantitative analysis of microRNA expression in three different types of cancer.
机构信息
Laboratory of Gene Engineering, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Department of the Structure and Function of Chromosomes, Laboratory of Molecular Genetics Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
出版信息
PLoS One. 2022 Feb 17;17(2):e0254304. doi: 10.1371/journal.pone.0254304. eCollection 2022.
MicroRNAs (miRNAs) are promising biomarkers in cancer research. Quantitative PCR (qPCR), also known as real-time PCR, is the most frequently used technique for measuring miRNA expression levels. The use of this technique, however, requires that expression data be normalized against reference genes. The problem is that a universal internal control for quantitative analysis of miRNA expression by qPCR has yet to be known. The aim of this work was to find the miRNAs with stable expression in the thyroid gland, brain and bone marrow according to NanoString nCounter miRNA quantification data. As a results, the most stably expressed miRNAs were as follows: miR-361-3p, -151a-3p and -29b-3p in the thyroid gland; miR-15a-5p, -194-5p and -532-5p in the brain; miR-140-5p, -148b-3p and -362-5p in bone marrow; and miR-423-5p, -28-5p and -532-5p, no matter what tissue type. These miRNAs represent promising reference genes for miRNA quantification by qPCR.
MicroRNAs (miRNAs) 是癌症研究中有前途的生物标志物。定量聚合酶链式反应 (qPCR)，也称为实时 PCR，是测量 miRNA 表达水平最常用的技术。然而，该技术的使用要求将表达数据与参考基因进行归一化。问题是，尚未确定用于 qPCR 定量分析 miRNA 表达的通用内部对照。本工作旨在根据 NanoString nCounter miRNA 定量数据，寻找在甲状腺、脑和骨髓中表达稳定的 miRNA。结果显示，在甲状腺中最稳定表达的 miRNA 为 miR-361-3p、-151a-3p 和 -29b-3p；在脑中有 miR-15a-5p、-194-5p 和 -532-5p；在骨髓中有 miR-140-5p、-148b-3p 和 -362-5p；无论组织类型如何，miR-423-5p、-28-5p 和 -532-5p 都有表达。这些 miRNA 代表用于 qPCR 定量分析 miRNA 的有前途的参考基因。
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