Thao Le Thi Thanh, Makkar Preeti, Tripathi Garima, Lee Byong-Taek
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, Cheonan, South Korea.
Institute of Tissue Regeneration, Soonchunhyang University, Cheonan, South Korea.
J Biomed Mater Res B Appl Biomater. 2022 Aug;110(8):1771-1779. doi: 10.1002/jbm.b.35033. Epub 2022 Feb 17.
Porous CDHA microspheres were incorporated into innovative injectable calcium phosphate cement (CPC) to enhance the rate of degradation and bioactivity of bone regeneration. With varying content of CDHA microspheres, the final setting time varied between 12 and 17 min, which is adequate for surgeons to accomplish the implantation. Compressive strength ranged between 6 and 8 MPa, until the addition of porous CDHA microsphere into CPC reached 20 vol %, but decreased dramatically after 30 vol % addition. Therefore, CPC with 20 vol % addition of porous CDHA microspheres was found appropriate for in vitro degradation and cytocompatibility studies. Histological assessment identified new bone formation around the injected bone substitute without significant inflammatory reactions. In vivo analysis of rat femoral defects revealed a threefold higher bone formation in CPC/CDHA 20 vol % than in CPC, due to the more cell migration and penetration into CPC by the existence of porous CDHA microspheres. Based on the promising results obtained, this novel injectable bone substitute may be useful in bone regeneration.
将多孔磷酸钙羟基磷灰石（CDHA）微球掺入创新的可注射磷酸钙骨水泥（CPC）中，以提高骨再生的降解速率和生物活性。随着CDHA微球含量的变化，最终凝固时间在12至17分钟之间变化，这足以让外科医生完成植入操作。抗压强度在6至8兆帕之间，直到向CPC中添加的多孔CDHA微球达到20体积%，但在添加量达到30体积%后急剧下降。因此，发现添加20体积%多孔CDHA微球的CPC适合进行体外降解和细胞相容性研究。组织学评估发现，注射的骨替代物周围有新骨形成，且无明显炎症反应。对大鼠股骨缺损的体内分析显示，由于多孔CDHA微球的存在，更多细胞迁移并渗透到CPC中，添加20体积% CDHA的CPC/CDHA组的骨形成量比单纯CPC组高三倍。基于所获得的有前景的结果，这种新型可注射骨替代物可能对骨再生有用。
