Department of Regenerative Medicine, College of Medicine, Soonchunhyang University 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, South Korea.
Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, South Korea.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109775. doi: 10.1016/j.msec.2019.109775. Epub 2019 May 18.
Bioglass-calcium phosphate cement (CPC) composite materials have recently received increased attention for bone regeneration purposes, owing to their improved properties in term of biocompatibility and bone ingrowths. In this study, an injectable bone substitute (IBS) system which utilizes bioglass microspheres incorporated into brushite based cement, was evaluated. The microspheres were synthesized with a simple and low sintering temperature process; there was no significant phase difference shown from the powder and good interactivity with cells was obtained. Furthermore, physical properties were optimized in microsphere incorporated brushite cement in order to investigate in vitro and in vivo performance. Accordingly, setting time and compressive strength were hardly altered until a microsphere content of 40% (v/v) was reached. The brushite (BR)/bioglass microsphere (BM) system showed excellent bioactivity to the in-vitro simulated body fluid test: dissolution ions from composite materials influenced apatite growth, countered acidic pH, and increased material degradation. In an in-vitro study with preosteoblasts (MC3T3-E1), BR/BM supported cell adhesion and proliferation, while cell differentiation experiments without osteogenic supplements, demonstrated that BR/BM induced osteogenic differentiation. A post-implantation study conducted in femoral defects showed higher materials degradation and bone formation in BR/BM than in BR. The faster dissolution of bioglass microspheres increased BR/BM composite resorption and hence facilitated bone tissue integration. Our findings suggest that bioglass microspheres incorporated in cement could potentially be used as an injectable bone substitute for bone regeneration applications.
生物玻璃-磷酸钙水泥（CPC）复合材料因其在生物相容性和骨长入方面的改善而受到越来越多的关注，目的是促进骨再生。在这项研究中，评估了一种利用掺入水合磷酸钙（brushite）水泥中的生物玻璃微球的可注射骨替代物（IBS）系统。这些微球是通过简单且低温烧结工艺合成的；从粉末中没有观察到明显的相差异，并获得了与细胞的良好相互作用。此外，为了研究体外和体内性能，对微球掺入水合磷酸钙水泥的物理性能进行了优化。相应地，直到达到 40%（v/v）的微球含量，凝固时间和抗压强度才几乎没有变化。水合磷酸钙（BR）/生物玻璃微球（BM）系统在体外模拟体液测试中表现出优异的生物活性：复合材料的溶解离子影响磷灰石的生长，中和酸性 pH 值，并增加材料降解。在与成骨前体细胞（MC3T3-E1）的体外研究中，BR/BM 支持细胞黏附和增殖，而在没有成骨补充剂的细胞分化实验中，证明 BR/BM 诱导成骨分化。在股骨干缺损的植入后研究中，BR/BM 中的材料降解和骨形成高于 BR。生物玻璃微球的更快溶解增加了 BR/BM 复合材料的吸收，从而促进了骨组织整合。我们的研究结果表明，掺入水泥中的生物玻璃微球可能有望作为用于骨再生应用的可注射骨替代物。
