Lee Soohyung, Jang Hwanhee, Yoo Inkyu, Han Jisoo, Jung Wonchul, Ka Hakhyun
Department of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
Asian-Australas J Anim Sci. 2019 Feb 9;32(9):1355-1362. doi: 10.5713/ajas.18.0920. Print 2019 Sep.
S100A7A, a member of the S100 protein family, is involved in various biological processes, including innate immunity, antimicrobial function, and epithelial tumorigenesis. However, the expression and function of S100A7A in the endometrium during the estrous cycle and pregnancy are not well understood in pigs. Therefore, this study determined the expression and regulation of S100A7A at the maternal-conceptus interface in pigs.
We obtained endometrial tissues from pigs throughout the estrous cycle and pregnancy, conceptus tissues during early pregnancy, and chorioallantoic tissues during mid- to late pregnancy and analyzed the expression of S100A7A in these tissues. We also determined the effects of steroid hormones, estradiol-17β (E2) and progesterone, and interleukin-1β (IL1B) on S100A7A expression in endometrial tissues.
We found that S100A7A was expressed in the endometrium during the estrous cycle and pregnancy in a pregnancy status- and stage-dependent manner and was localized to endometrial LE and superficial GE cells with strong intensity in LE cells on Day 12 of pregnancy. Early stage conceptuses and chorioallantoic tissues from Day 30 to term pregnancy also expressed S100A7A. The expression of S100A7A was increased by E2 and IL1B in endometrial tissues.
S100A7A was expressed at the maternal-conceptus interface at the initiation of implantation in response to conceptus-derived estrogen and IL1B and could be a unique endometrial epithelial marker for conceptus implantation in pigs. These findings provide an important insight into the understanding of conceptus-endometrial interactions for the successful establishment of pregnancy in pigs.
S100A7A是S100蛋白家族的成员，参与多种生物学过程，包括先天免疫、抗菌功能和上皮肿瘤发生。然而，猪发情周期和妊娠期子宫内膜中S100A7A的表达和功能尚不清楚。因此，本研究确定了猪母胎界面处S100A7A的表达及调控。
我们获取了猪发情周期和妊娠期的子宫内膜组织、妊娠早期的胎体组织以及妊娠中后期的绒毛膜尿囊组织，并分析了这些组织中S100A7A的表达。我们还确定了类固醇激素雌二醇-17β（E2）、孕酮以及白细胞介素-1β（IL1B）对子宫内膜组织中S100A7A表达的影响。
我们发现，S100A7A在猪发情周期和妊娠期的子宫内膜中表达，呈现出与妊娠状态和阶段相关的方式，并且定位于子宫内膜腔上皮（LE）和浅表腺上皮（GE）细胞，在妊娠第12天LE细胞中的表达强度较强。早期胎体以及妊娠第30天至足月的绒毛膜尿囊组织也表达S100A7A。E2和IL1B可增加子宫内膜组织中S100A7A的表达。
S100A7A在着床起始时响应胎体来源的雌激素和IL1B，在母胎界面表达，可能是猪胎体着床的一种独特的子宫内膜上皮标志物。这些发现为理解猪成功建立妊娠过程中胎体与子宫内膜的相互作用提供了重要见解。
