Peng Mei-Mei, Guo Shuang, Wang Lu, Mao Jing, Xie Hui, Zhao Xiao-Li, Hou Jin-Cai, Guo Yong, Lu Tu-Lin, Mao Chun-Qin
School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
Jiangsu Province Hospital of Chinese Medicine Nanjing 210029, China.
Zhongguo Zhong Yao Za Zhi. 2022 Jan;47(2):313-323. doi: 10.19540/j.cnki.cjcmm.20210412.304.
Following the preparation of substance benchmarks in Huanglian Decoction from 18 batches, the method for detecting their characteristic spectra was established to identify the similarity range and peak attribution. The content and transfer rate ranges of the index components coptisine, palmatine, berberine, liquiritin, glycyrrhizic acid, 6-gingerol, and cinnamaldehyde and the extraction amount were combined for analyzing the quality value transfer from the Chinese medicinal pieces to substance benchmarks and clarifying the key quality attributes of substance benchmarks in Huanglian Decoction. The results showed that the substance benchmarks in Huang-lian Decoction of 18 batches exhibited good similarity in characteristic spectra(all greater than 0.98). There were 17 characteristic peaks identified in the substance benchmarks of Huanglian Decoction, including 10 from Coptidis Rhizoma, 3 from Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle(processed with water), 1 from Zingiberis Rhizoma, and 3 from Cinnamomi Ramulus. The contents and average transfer rates of the index components were listed as follows: coptisine 2.20-6.46 mg·g(-1) and 18.50%±2.93%; palmatine 3.03-8.13 mg·g(-1) and 26.56%±4.69%; berberine 7.71-22.29 mg·g(-1) and 17.34%±3.00%; liquiritin 0.88-2.18 mg·g(-1) and 9.88%±4.88%; glycyrrhizic acid 1.83-4.44 mg·g(-1) and 8.50%±3.72%; 6-gingerol 0.56-1.43 mg·g(-1) and 11.36%±2.37%; cinnamaldehyde 1.55-3.48 mg·g~(-1) and 19.02%±4.36%. The extraction amount of the substance benchmarks from the 18 batches was controlled at 10.65%-13.88%. In this paper, the quality value transfer of substance benchmarks in Huanglian Decoction was analyzed based on the characteristic spectra, the index component contents and the extraction amount, which has provided a basis for the subsequent development of Huanglian Decoction and the quality control of its related preparations.
在制备18批次黄连汤物质基准后，建立了其特征图谱检测方法，以确定相似度范围和峰归属。结合指标成分黄连碱、巴马汀、小檗碱、甘草苷、甘草酸、6-姜酚和桂皮醛的含量及转移率范围以及提取量，分析了中药饮片到物质基准的质量值传递情况，明确了黄连汤物质基准的关键质量属性。结果表明，18批次黄连汤物质基准的特征图谱相似度良好（均大于0.98）。黄连汤物质基准中鉴定出17个特征峰，其中10个来自黄连，3个来自蜜甘草，1个来自干姜，3个来自桂枝。指标成分的含量及平均转移率如下：黄连碱2.20 - 6.46 mg·g⁻¹，转移率18.50%±2.93%；巴马汀3.03 - 8.13 mg·g⁻¹，转移率26.56%±4.69%；小檗碱7.71 - 22.29 mg·g⁻¹，转移率17.34%±3.00%；甘草苷0.88 - 2.18 mg·g⁻¹，转移率9.88%±4.88%；甘草酸1.83 - 4.44 mg·g⁻¹，转移率8.50%±3.72%；6-姜酚0.56 - 1.43 mg·g⁻¹，转移率11.36%±2.37%；桂皮醛1.55 - 3.48 mg·g⁻¹，转移率19.02%±4.36%。18批次物质基准的提取量控制在10.65% - 13.88%。本文基于特征图谱、指标成分含量和提取量分析了黄连汤物质基准的质量值传递情况，为黄连汤后续开发及其相关制剂的质量控制提供了依据。
