Liu Xue-Chun, Mo Yu-Jia, Zhang Qing, Hu Wen-Jun, Shen Ruo-Nan, Zhao Yue-Ying, Zhang Ze-Kang, Wang Chang-Hai, Huang Xing-Yue, Lu Yang, Bai Jie
School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China Shenzhen Research Institute Beijing University of Chinese Medicine Shenzhen 518000, China.
Zhongguo Zhong Yao Za Zhi. 2022 Apr;47(8):2099-2108. doi: 10.19540/j.cnki.cjcmm.20210901.302.
According to the polarity of different components in Sanpian Decoction, two fingerprints were established. Then the substance benchmark freeze-dried powder of 15 batches of Sanpian Decoction was prepared, followed by the determination of the fingerprints, index component content, and dry extract rates, the identification of attribution of characteristic peaks, and the calculation of similarities between these fingerprints and the reference(R), the content and transfer rate ranges of ferulic acid, sinapine thiocyanate, liquiritin, and glycyrrhizic acid, and the dry extract rate range. The results showed that the similarities of 15 batches of the substance benchmark fingerprints with R were all greater than 0.900.Further summarization of the characteristic peaks revealed that there were a total of 20 characteristic peaks in fingerprint 1, among which, eight were from Sinapis Semen, four from Paeoniae Radix Alba, six from Chuanxiong Rhizoma, and two from Glycyrrhizae Radix et Rhizoma. A total of 16 characteristic peaks were observed in fingerprint 2, including one from Sinapis Semen, three from Paeoniae Radix Alba, eight from Chuanxiong Rhizoma, and four from Glycyrrhizae Radix et Rhizoma. The average dry extract rate of 15 batches of substance benchmarks was 18.25%, with a dry extract rate range of 16.28%-20.76%. The index component content and transfer rate ranges were listed as follows: 0.15%-0.18% and 38.81%-58.05% for ferulic acid; 0.26%-0.42% and 36.51%-51.02% for sinapine thiocyanate; 0.09%-0.15% and 48.80%-76.61% for liquiritin; 0.13%-0.24% and 23.45%-35.61% for glycyrrhizic acid. The fingerprint, dry extract rate, and index component content determination was combined for analyzing the quality value transfer of substance benchmarks in the classic prescription Sanpian Decoction.The established quality evaluation method for the substance benchmarks was stable and feasible, which has provided a basis for the quality control of Sanpian Decoction and the follow-up development of related preparations.
根据三痹汤中不同成分的极性，建立了两个指纹图谱。然后制备了15批次三痹汤的物质基准冻干粉，接着测定指纹图谱、指标成分含量和干浸膏得率，鉴定特征峰归属，计算这些指纹图谱与对照图谱（R）之间的相似度、阿魏酸、芥子碱硫氰酸盐、甘草苷和甘草酸的含量及转移率范围以及干浸膏得率范围。结果表明，15批次物质基准指纹图谱与R的相似度均大于0.900。进一步总结特征峰发现，指纹图谱1共有20个特征峰，其中，来源于白芥子的有8个，来源于白芍的有4个，来源于川芎的有6个，来源于甘草的有2个。指纹图谱2共观察到16个特征峰，包括来源于白芥子的1个，来源于白芍的3个，来源于川芎的8个，来源于甘草的4个。15批次物质基准的平均干浸膏得率为18.25%，干浸膏得率范围为16.28% - 20.76%。指标成分含量及转移率范围如下：阿魏酸为0.15% - 0.18%和38.81% - 58.05%；芥子碱硫氰酸盐为0.26% - 0.42%和36.51% - 51.02%；甘草苷为0.09% - 0.15%和48.80% - 76.61%；甘草酸为0.13% - 0.24%和23.45% - 35.61%。将指纹图谱、干浸膏得率和指标成分含量测定相结合，分析经典方剂三痹汤物质基准的质量值传递情况。所建立的物质基准质量评价方法稳定可行，为三痹汤的质量控制及相关制剂的后续开发提供了依据。
Zotero 插件