School of Veterinary Science, University of Queensland, Gatton, QLD, Australia (Sprohnle-Barrera, Gibson, Price, Allavena).
Public Health Microbiology, Queensland Reference Centre for Microbial and Public Health Genomics (MPHG), Forensic and Scientific Services, Health Support Queensland, Queensland Health, Brisbane, QLD, Australia (Graham, Jennison).
J Vet Diagn Invest. 2022 May;34(3):518-522. doi: 10.1177/10406387221079066. Epub 2022 Feb 18.
An 8-y-old, castrated male Siberian Husky dog was admitted to an emergency clinic with acute collapse and severe swelling of both forelimbs, ventral thorax, and axillary region. The clinical assessment, with laboratory tests and radiologic investigation, confirmed severe subcutaneous emphysema and multi-organ failure. The animal died while receiving emergency treatment. On postmortem examination, was isolated from the subcutaneous fluid and the effusion from the thoracic and abdominal cavities. Relevant histopathology findings included subcutaneous emphysema and multi-organ perivascular and intravascular, intralesional myriad 0.5-3-µm gram-positive rod bacteria, with no associated inflammation. Whole-genome sequencing and phylogenetic analysis identified type A. Virulence genes detected included (alpha toxin), (v-toxin), (collagenase A), (hyaluronidase), , , (sialidases), and (perfringolysin). These virulence genes have previously been reported to act synergistically with alpha toxin in mediated gas gangrene.
一只 8 岁、已去势的雄性西伯利亚哈士奇犬因四肢前肢、胸腹部和腋窝急性肿胀而被紧急送往诊所。临床评估、实验室检查和影像学研究均证实存在严重皮下气肿和多器官衰竭。该动物在接受紧急治疗时死亡。在尸检时，从皮下积液和胸腔、腹腔渗出液中分离出。相关组织病理学发现包括皮下气肿和多器官血管周围和血管内、病变内存在无数 0.5-3-µm 革兰氏阳性棒状细菌，没有伴随炎症。全基因组测序和系统发育分析鉴定为 A 型。检测到的毒力基因包括 （α毒素）、 （v 毒素）、 （胶原酶 A）、 （透明质酸酶）、 、 （唾液酸酶）和 （产气荚膜梭菌溶细胞素）。这些毒力基因以前曾被报道与 α 毒素协同作用，导致气性坏疽。
