van der Laan-Klamer S M, Harms G, Atmosoerodjo-Briggs J, Hoedemaeker P J, Hardonk M J
Scand J Immunol. 1986 Apr;23(4):441-7.
The hepatic uptake of autologous immune complexes (ICx) was studied in Wistar rats immunized with bovine serum albumin (BSA). After attaining a suitable serum titre of rat anti-BSA Ig (RatABSA) they were injected intravenously with 2 mg BSA. The antibody levels were measured with an ELISA and the rate of disappearance of BSA from the circulation was studied by labelling the antigen with FITC. At various times after injection of BSA the uptake of ICx in the different types of liver cells was studied with the immunoperoxidase technique at the light microscope and ultrastructural levels. Within 5 min of injection with BSA ICx appeared to be bound and internalized by Kupffer cells, endothelial cells, and hepatocytes. After 2 h hepatocytes showed many large vacuoles containing ICx, whereas most microvilli had disappeared from the sinusoidal plasma membrane of these cells. In rats with a high antibody titre BSA had disappeared almost completely (92%) from the blood within 2 h. Rats with low antibody titre showed a lower disappearance rate of BSA, whereas a considerable amount of antibody was still present in the blood after 2 h. We conclude that autologous ICx are bound and endocytosed by Kupffer cells, endothelial cells and hepatocytes of the liver. The affinity for the antigen from the circulation. Uptake of autologous ICx by hepatocytes occurs only in the presence of large amounts of circulating ICx.
在以牛血清白蛋白（BSA）免疫的Wistar大鼠中研究了自体免疫复合物（ICx）的肝脏摄取情况。在大鼠抗牛血清白蛋白Ig（RatABSA）达到合适的血清滴度后，给它们静脉注射2mg BSA。用酶联免疫吸附测定法（ELISA）测量抗体水平，并用异硫氰酸荧光素（FITC）标记抗原研究BSA从循环中的消失速率。在注射BSA后的不同时间，用免疫过氧化物酶技术在光学显微镜和超微结构水平研究不同类型肝细胞中ICx的摄取情况。注射BSA后5分钟内，ICx似乎被库普弗细胞、内皮细胞和肝细胞结合并内化。2小时后，肝细胞显示出许多含有ICx的大液泡，而这些细胞的窦状质膜上的大多数微绒毛已经消失。在抗体滴度高的大鼠中，BSA在2小时内几乎完全（92%）从血液中消失。抗体滴度低的大鼠中，BSA的消失速率较低，而2小时后血液中仍存在相当数量的抗体。我们得出结论，自体ICx被肝脏的库普弗细胞、内皮细胞和肝细胞结合并内吞。对循环中抗原的亲和力。肝细胞对自体ICx的摄取仅在存在大量循环ICx的情况下发生。
