College of Tropical Crops, Hainan University, Haikou, P.R. China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, P.R. China.
GM Crops Food. 2022 Dec 31;13(1):1-14. doi: 10.1080/21645698.2022.2025725. Epub 2022 Feb 18.
The deliberate introduction of the beneficial gene in crop plants through transgenic technology can provide enormous agricultural and economic benefits. However, the impact of commercialization of these crops on the ecosystem particularly on belowground soil biodiversity is still uncertain. Here, we examined and compared the effects of a non-transgenic maize cultivar and an insect-resistant transgenic maize cultivar genetically engineered with gene from , on the rhizosphere bacterial community using 16S rDNA amplicon sequencing and soil metabolome profile using UPLC/MS analysis at six different growth stages. We found no significant differences in bacterial community composition and diversity at all growth stages between the two cultivars. The analysis of bacterial beta-diversity showed an evident difference in community structure attributed to plant different growth stages but not to the plant type. In contrast, the soil metabolic profile of transgenic maize differed from that of the non-transgenic plant at some growth stages, and most of the altered metabolites were usually related to the metabolism but not to the plant-microbe interaction related pathways. These results suggest that genetic modification with the gene-altered maize soil metabolism but had no obvious effect on the rhizosphere bacterial community.
通过转基因技术有意将有益基因引入作物中，可以带来巨大的农业和经济效益。然而，这些作物商业化对生态系统的影响，特别是对地下土壤生物多样性的影响仍不确定。在这里，我们使用 16S rDNA 扩增子测序和 UPLC/MS 分析检测并比较了非转基因玉米品种和转 Bt 基因抗虫玉米品种（转自 ）在六个不同生长阶段对根际细菌群落的影响。我们发现，在所有生长阶段，两种玉米品种的细菌群落组成和多样性均无显著差异。细菌β多样性分析表明，由于植物不同的生长阶段，而不是由于植物类型，群落结构存在明显差异。相比之下，在某些生长阶段，转基因玉米的土壤代谢谱与非转基因植物不同，而大多数改变的代谢物通常与代谢有关，而与植物-微生物相互作用相关途径无关。这些结果表明，基因修饰的转 基因玉米改变了土壤代谢，但对根际细菌群落没有明显影响。
