Zhang Xuejing, Wang Tai, Zhai Dongdong, Liu Hongyan, Xiong Fei, Wang Ying
Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, Jianghan University, Wuhan, China.
Gansu Key Laboratory of Cold Water Fishes Germplasm Resources and Genetics Breeding, Gansu Fishers Research Institute, Lanzhou, China.
Front Genet. 2023 Jan 12;13:1089274. doi: 10.3389/fgene.2022.1089274. eCollection 2022.
is a common species of , and it is distributed in Shule river of Hexi Corridor, of Gansu province in China. In order to enrich gene database resources and explore the environment adaptation of , fifteen tissues were collected from three adult for transcriptome sequencing and assembly. Nine major international gene annotation databases (NR, COG, egg_NOG, TrEMBL, Pfam, KOG, Swiss prot, KEGG and Gene Ontology) were utilized to annotate unigenes. A detailed study was conducted to explore the gene expression and the differentially expressed genes among five tissues (brain, heart, kidney, liver and spleen). In addition, the current study showed that candidate genes involved in salinity-alkalinity and low temperature adaptation were differentially expressed in tissues of . Precisely, , , , , and genes participated in the regulation process of salinity-alkalinity adaptation, and , , , , and genes were involved in fatty acid metabolism and closely associated with low temperature adaptation. On the one hand, it was found that the expression of these genes varied among different tissues, and the important pathways involved in these genes were mapped. Furthermore, we analyzed and genes in depth to obtain the predicted gene structure and important amino acid sites. The transcriptome information in this study will be conducive to provide further understanding for the molecular level research and exploration of the environmental adaptation of .
是一种常见的 物种，分布于中国甘肃省河西走廊的疏勒河。为了丰富基因数据库资源并探索 的环境适应性，从三只成年 身上采集了十五个组织进行转录组测序和 组装。利用九个主要的国际基因注释数据库（NR、COG、egg_NOG、TrEMBL、Pfam、KOG、Swiss prot、KEGG和基因本体论）对单基因进行注释。开展了一项详细研究，以探索五个组织（脑、心脏、肾脏、肝脏和脾脏）中的基因表达及差异表达基因。此外，当前研究表明，参与盐碱和低温适应的候选基因在 的组织中差异表达。具体而言， 、 、 、 、 和 基因参与了盐碱适应的调控过程， 、 、 、 、 和 基因参与脂肪酸代谢且与低温适应密切相关。一方面，发现这些基因在不同组织中的表达存在差异，并绘制了这些基因涉及的重要途径。此外，我们深入分析了 和 基因，以获得预测的基因结构和重要氨基酸位点。本研究中的转录组信息将有助于为 的环境适应性分子水平研究和探索提供进一步的理解。
