Biological Applications Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Abu-Zaabal, 13759, Egypt.
Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, 11651, Egypt.
J Therm Biol. 2022 Feb;104:103195. doi: 10.1016/j.jtherbio.2022.103195. Epub 2022 Jan 29.
This study was conducted to assess the impact of dietary incorporation of Spirulina platensis and selenium nanoparticles (SeNPs) individually or in combinations on growth performance, antioxidant status, humoral immune response, and microbial populations in diet and ileum of heat-stressed broilers. Ross-308 one-day chicks (n = 450) were fed one of 9 experimental diets with five replicate cages in 2 phases for 35 d. The experimental diets were a control basal diet without supplementation or with 0.1 mg SeNPs, 0.2 mg SeNPs, 5 g Spirulina, 10 g Spirulina, 0.1 mg SeNPs + 5 g Spirulina, 0.1 mg SeNPs + 10 g Spirulina, 0.2 mg SeNPs + 5 g Spirulina and 0.2 mg SeNPs + 10 g Spirulina per kg diet. Dietary supplementation with Spirulina and SeNPs significantly (P < 0.05) increased body weight gain and European production efficiency factor. Serum GPx and SOD were significantly (P < 0.05) increased with dietary Spirulina and SeNPs supplementation, while, TBARS was decreased (P < 0.05). Circulating immunoglobulin IgM, IgA and IgG were increased in treated birds compared to the control ones, while the antibody titers to IBD, AIV, and NDV were not significantly altered. The results showed that SeNPs and Spirulina exhibited dose-dependent antimicrobial activities against ileal counts of total bacterial, total molds and yeast, coliform, E. coli, Salmonella spp. and Enterococcus spp. However, ileal populations of Lactic acid bacteria were increased with dietary Spirulina and SeNPs in a dose-dependent manner. The microbial load in broilers' diets was reduced by dietary incorporation of S. platensis and SeNPs. These results indicate that Spirulina and SeNPs can be potentially used as growth promoters and antioxidant, immunostimulant, and antimicrobial agents in heat-stressed broilers.
本研究旨在评估螺旋藻和硒纳米粒子（SeNPs）单独或组合添加到饲料中对热应激肉鸡生长性能、抗氧化状态、体液免疫反应和肠道微生物区系的影响。罗斯 308 一日龄雏鸡（n=450）分为 9 个实验组，每个实验组设 5 个重复，饲养 35d。实验日粮分为对照组（不添加任何物质）、0.1mg/kg SeNPs、0.2mg/kg SeNPs、5g/kg 螺旋藻、10g/kg 螺旋藻、0.1mg/kg SeNPs+5g/kg 螺旋藻、0.1mg/kg SeNPs+10g/kg 螺旋藻、0.2mg/kg SeNPs+5g/kg 螺旋藻和 0.2mg/kg SeNPs+10g/kg 螺旋藻。日粮添加螺旋藻和 SeNPs 可显著（P<0.05）提高肉鸡的体重和欧洲生产效率系数。血清谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）活性随螺旋藻和 SeNPs 日粮添加而显著（P<0.05）升高，而丙二醛（TBARS）含量则降低（P<0.05）。与对照组相比，处理组鸡的循环免疫球蛋白 IgM、IgA 和 IgG 均升高，而对 IBD、AIV 和 NDV 的抗体滴度无显著影响。结果表明，SeNPs 和螺旋藻对回肠总细菌、总霉菌和酵母、大肠菌群、大肠杆菌、沙门氏菌和肠球菌计数具有剂量依赖性的抗菌活性。然而，日粮添加螺旋藻和 SeNPs 可使肠道乳酸菌数量呈剂量依赖性增加。螺旋藻和 SeNPs 可降低饲料中的微生物负荷。这些结果表明，螺旋藻和 SeNPs 可作为生长促进剂和抗氧化剂、免疫刺激剂以及抗菌剂在热应激肉鸡中使用。
