Clinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain; Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain.
Clinical Immunology Laboratory, Veterinary Faculty, University of Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain.
Acta Trop. 2022 May;229:106371. doi: 10.1016/j.actatropica.2022.106371. Epub 2022 Feb 16.
Canine leishmaniosis is a vector-borne disease caused by Leishmania parasites. Serological methods are the most common tests used for the diagnosis. This study aimed to evaluate and compare different serological commercial immunochromatographic rapid tests available in Spain to detect anti-Leishmania canine antibodies. The immunochromatographic tests were evaluated in different groups of dogs (healthy seronegative dogs (n = 21), naturally-sick dogs with moderate anti-Leishmania antibodies (n = 39), naturally-sick dogs with high anti-Leishmania antibodies (n = 37), dogs with the serological result of other pathogens infection (n = 20) and exposed dogs (n = 33)) admitted to the Veterinary Teaching Hospital of the University of Zaragoza (Spain) according to the clinical information sent with the sample to the laboratory for diagnostic purposes. The serology status was also routinely recorded through an in-house enzyme-linked immunosorbent assay (ELISA) and an in-house indirect immunofluorescence test (IFAT). The qualitative commercial serological immunochromatographic tests used were: FASTest LEISH, Uranotest Leishmania, Uranotest Leishmania 2.0, Speed Leish K, Witness Leishmania, and DFV Test Leishmania. Performance measures analyzed for each test were: sensitivity, specificity, and area under the receiver-operating (ROC) curve. The maximum specificity (1.00) was attained for Uranotest Leishmania and DFT Test Leishmania, followed by FASTest LEISH (0.98), Uranotest Leishmania 2.0 (0.98), Speed Leish K (0.98), and Witness Leishmania (0.95). The maximum sensitivity was attained for FASTest LEISH (1.00), followed by Uranotest 2.0 (0.97), Speed Leish K (0.97), Uranotest (0.96), and the lowest results with Witness (0.84) and DFV Test (0.59). Regarding the ROC curve, the maximum value was attained with the FASTest LEISH (0.99), followed by Uranotest (0.98), Uranotest 2.0 (0.97), Speed Leish K (0.97), Witness (0.90), and the lowest result with DFV Test (0.79). Efforts in the field of diagnosis should focus on establishing a commercial immunochromatographic test with high sensitivity and specificity with a reasonable cost-benefit balance.
犬利什曼病是一种由利什曼原虫寄生虫引起的虫媒病。血清学方法是最常用于诊断的检测方法。本研究旨在评估和比较西班牙市售的不同利什曼原虫犬抗体检测的免疫层析快速检测试剂盒。免疫层析检测试剂盒用于评估不同组别犬的检测结果：健康的血清阴性犬（n=21）、有中度利什曼抗体的自然感染犬（n=39）、有高滴度利什曼抗体的自然感染犬（n=37）、有其他病原体感染的血清学结果的犬（n=20）和暴露犬（n=33）。这些犬的临床信息与样本一起被送到兽医教学医院（西班牙萨拉戈萨）的实验室进行诊断。血清学状态也通过内部酶联免疫吸附试验（ELISA）和内部间接免疫荧光试验（IFAT）进行常规记录。使用的商业免疫层析血清学检测试剂盒包括：FASTest LEISH、Uranotest Leishmania、Uranotest Leishmania 2.0、Speed Leish K、Witness Leishmania 和 DFV Test Leishmania。对每个检测试剂盒的分析性能指标包括：敏感性、特异性和接受者操作特征（ROC）曲线下的面积。Uranotest Leishmania 和 DFT Test Leishmania 的最大特异性（1.00），其次是 FASTest LEISH（0.98）、Uranotest Leishmania 2.0（0.98）、Speed Leish K（0.98）和 Witness Leishmania（0.95）。FASTest LEISH 的最大敏感性为 1.00，其次是 Uranotest 2.0（0.97）、Speed Leish K（0.97）、Uranotest（0.96），最低的是 Witness（0.84）和 DFV Test（0.59）。关于 ROC 曲线，FASTest LEISH 的最大值为 0.99，其次是 Uranotest（0.98）、Uranotest 2.0（0.97）、Speed Leish K（0.97）、Witness（0.90），DFV Test 的结果最低（0.79）。诊断领域的工作重点应放在建立具有高灵敏度和特异性的商业免疫层析检测试剂盒，同时平衡成本效益。
