Fox J C, Jordan H E, Kocan K M, George T J, Mullins S T, Barnett C E, Glenn B L, Cowell R L
Vet Parasitol. 1986 Mar;20(1-3):13-29. doi: 10.1016/0304-4017(86)90089-0.
Current methods and commercial test systems for the diagnosis of parasitic infections in both animals and humans are reviewed. Lists of test kits and their manufacturers are provided along with ordering information: the only commercially available test kits are for the diagnosis of toxoplasmosis in humans or animals and dirofilariasis (heartworm) in dogs. A partial list of diagnostic laboratories and the parasite tests they perform is also provided. Complete lists of diagnostic tests that could be obtained in the private sector are not available but would be useful. Two microfluorometric solid-phase assay systems are reviewed, and adaptations to custom assays for several kinds of parasites are briefly described. User problems in performing tests and interpreting results are stressed with emphasis placed on diagnosis of dirofilariasis in dogs. False-positive serology in dogs without heartworms and negative antibody responses in micro-filariae-positive animals are discussed with respect to proper interpretation of results.
本文综述了目前用于诊断动物和人类寄生虫感染的方法及商业检测系统。文中提供了检测试剂盒及其制造商列表以及订购信息:唯一可商购的检测试剂盒用于诊断人类或动物的弓形虫病以及犬类的心丝虫病(恶丝虫病)。此外,还提供了一份诊断实验室及其所开展的寄生虫检测项目的部分清单。虽无法获取私营部门可提供的完整诊断检测清单,但该清单会很有用。本文对两种微荧光固相检测系统进行了综述,并简要描述了针对几种寄生虫定制检测方法的调整情况。文中强调了用户在进行检测和解读结果时遇到的问题,重点在于犬类心丝虫病的诊断。针对无心丝虫犬类血清学检测出现假阳性以及微丝蚴阳性动物抗体反应呈阴性的情况,文中讨论了如何正确解读检测结果。