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目前可用于寄生虫感染实验室诊断的血清学检测概述。

An overview of serological tests currently available for laboratory diagnosis of parasitic infections.

作者信息

Fox J C, Jordan H E, Kocan K M, George T J, Mullins S T, Barnett C E, Glenn B L, Cowell R L

出版信息

Vet Parasitol. 1986 Mar;20(1-3):13-29. doi: 10.1016/0304-4017(86)90089-0.

DOI:10.1016/0304-4017(86)90089-0
PMID:3518213
Abstract

Current methods and commercial test systems for the diagnosis of parasitic infections in both animals and humans are reviewed. Lists of test kits and their manufacturers are provided along with ordering information: the only commercially available test kits are for the diagnosis of toxoplasmosis in humans or animals and dirofilariasis (heartworm) in dogs. A partial list of diagnostic laboratories and the parasite tests they perform is also provided. Complete lists of diagnostic tests that could be obtained in the private sector are not available but would be useful. Two microfluorometric solid-phase assay systems are reviewed, and adaptations to custom assays for several kinds of parasites are briefly described. User problems in performing tests and interpreting results are stressed with emphasis placed on diagnosis of dirofilariasis in dogs. False-positive serology in dogs without heartworms and negative antibody responses in micro-filariae-positive animals are discussed with respect to proper interpretation of results.

摘要

本文综述了目前用于诊断动物和人类寄生虫感染的方法及商业检测系统。文中提供了检测试剂盒及其制造商列表以及订购信息:唯一可商购的检测试剂盒用于诊断人类或动物的弓形虫病以及犬类的心丝虫病(恶丝虫病)。此外,还提供了一份诊断实验室及其所开展的寄生虫检测项目的部分清单。虽无法获取私营部门可提供的完整诊断检测清单,但该清单会很有用。本文对两种微荧光固相检测系统进行了综述,并简要描述了针对几种寄生虫定制检测方法的调整情况。文中强调了用户在进行检测和解读结果时遇到的问题,重点在于犬类心丝虫病的诊断。针对无心丝虫犬类血清学检测出现假阳性以及微丝蚴阳性动物抗体反应呈阴性的情况,文中讨论了如何正确解读检测结果。

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An overview of serological tests currently available for laboratory diagnosis of parasitic infections.目前可用于寄生虫感染实验室诊断的血清学检测概述。
Vet Parasitol. 1986 Mar;20(1-3):13-29. doi: 10.1016/0304-4017(86)90089-0.
2
[The serodiagnosis of parasitic infections].[寄生虫感染的血清学诊断]
Parassitologia. 2004 Jun;46(1-2):141-4.
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Comparison of results of three commercial heartworm antigen test kits in dogs with low heartworm burdens.三种市售犬心丝虫抗原检测试剂盒在低心丝虫感染负担犬只中的检测结果比较。
J Am Vet Med Assoc. 2003 May 1;222(9):1221-3. doi: 10.2460/javma.2003.222.1221.
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Prime detection of Dirofilaria immitis: understanding the influence of blocked antigen on heartworm test performance.早期检测犬心丝虫:了解被阻断抗原对心丝虫检测性能的影响。
Parasit Vectors. 2018 Mar 20;11(1):186. doi: 10.1186/s13071-018-2736-5.
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Comparison of serological tests for the detection of natural heartworm infection in cats.
J Am Anim Hosp Assoc. 2004 Sep-Oct;40(5):376-84. doi: 10.5326/0400376.
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[Problems and limitations of conventional and innovative methods for the diagnosis of Toxoplasmosis in humans and animals].[人类和动物弓形虫病诊断的传统方法与创新方法的问题及局限性]
Parassitologia. 2004 Jun;46(1-2):177-81.
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Comparison of serologic tests for detection of antigen in canine heartworm infections.犬心丝虫感染中用于检测抗原的血清学检测方法比较
J Am Vet Med Assoc. 1988 May 15;192(10):1423-7.
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Serologic and intradermal tests for parasitic infections.针对寄生虫感染的血清学和皮内试验。
Pediatr Clin North Am. 1985 Aug;32(4):1063-75. doi: 10.1016/s0031-3955(16)34870-2.
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Comparison of six commercial antigen kits for detection of Dirofilaria immitis infections in canines with necropsy-confirmed heartworm status.六种用于检测犬类心丝虫感染的商业抗原检测试剂盒与尸检确诊的心丝虫感染状况的比较。
Vet Parasitol. 2018 Apr 30;254:178-182. doi: 10.1016/j.vetpar.2018.02.037. Epub 2018 Feb 25.
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Serodiagnosis of Toxoplasma gondii infection in farm animals (horses, swine, and sheep) by enzyme-linked immunosorbent assay using chimeric antigens.使用嵌合抗原通过酶联免疫吸附测定法对农场动物(马、猪和羊)弓形虫感染进行血清学诊断。
Parasitol Int. 2015 Oct;64(5):288-94. doi: 10.1016/j.parint.2015.03.004. Epub 2015 Mar 25.

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