用胎儿三毛滴虫疫苗接种牛后IgG抗体反应的荧光测定
Fluorometric measurement of IgG antibody responses in cattle vaccinated with Tritrichomonas foetus.
作者信息
Gorton K A, Fulton R W, Fox J C, Rice L E
机构信息
Department of Veterinary Parasitology, Microbiology and Public Health, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
出版信息
Can J Vet Res. 1993 Apr;57(2):114-8.
A photometrically-measured indirect fluorescent antibody (IFA) test was developed to measure antibody levels in three heifers vaccinated intramuscularly with two injections (two-week interval) of a vaccine containing formalin-killed Tritrichomonas foetus in oil adjuvant; a separate animal served as a nonvaccinated control. Sera were collected weekly and tested for specific IgG against T. foetus. Antibodies were detectable within two weeks of the initial injection and reached reciprocal titers as high as 7700 as estimated from previously tested reference sera. Titers peaked at six to eight weeks and remained at relatively high levels for the eleven-week study period. The fluorometric assay was easily developed and economical to perform; in addition, it more accurately estimated IgG levels than standard slide IFA tests.
开发了一种通过光度测量的间接荧光抗体（IFA）试验，以测量三头经肌肉注射两剂（间隔两周）含油佐剂中福尔马林灭活胎儿三毛滴虫疫苗的小母牛的抗体水平；另一只动物作为未接种疫苗的对照。每周采集血清并检测针对胎儿三毛滴虫的特异性IgG。在初次注射后两周内可检测到抗体，根据先前检测的参考血清估计，抗体效价高达7700。效价在六至八周达到峰值，并在为期十一周的研究期间保持在相对较高的水平。荧光测定法易于开发且执行成本低；此外，与标准玻片IFA试验相比，它能更准确地估计IgG水平。
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