Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour, 22511, Egypt.
Molecular Biology, Molecular biology unit, Medical Technology Center, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Environ Sci Pollut Res Int. 2022 Jul;29(31):47436-47447. doi: 10.1007/s11356-022-18984-8. Epub 2022 Feb 19.
Aging represents the accumulation of progressive changes in a human being over time and can cover physical, psychological, and social changes. It is an oxidative stress-associated process that progresses with age. The antioxidant activity of either eugenol (EU) or carvacrol (CAR) for aging in rats induced by D-gal for 42 days was investigated in the current study using 10 and 20 mg of EU/kg/day/orally, while CAR was supplemented by 40 and 80 mg /kg/day/orally. Biochemical, mRNA expression, and histopathological assessments of brain samples evaluated the oxidative alterations induced by D-gal and the protective role of EU and CAR. Results showed that D-gal was causing oxidative alternation of the brain that was recognized via upregulation of p53 and p21 mRNA expression levels, as aging markers and Bax mRNA expression level, as an apoptotic marker. Also, the results observed alterations in the levels of biochemical markers as creatine phosphokinase (CPK) and triacylglycerol (TAG), besides, enhancement of brain antioxidant capacity. Finally, these results compared with the groups treated with EU and CAR to observe that the EU and CAR potentially attenuate these aging-related oxidative alterations in a dose-dependent manner. Finally, we can conclude that EU and CAR supplementations are considered promising natural protective compounds that could delay aging and maintain health.
衰老是指人类随着时间的推移而积累的渐进性变化，可以包括身体、心理和社会变化。它是一个与氧化应激相关的过程，随着年龄的增长而进展。本研究旨在探讨丁香酚（EU）或香芹酚（CAR）对 D-半乳糖诱导的大鼠衰老的抗氧化作用，为期 42 天，每天用 10 和 20mg/kg 经口给予 EU，每天用 40 和 80mg/kg 经口给予 CAR。通过评估大脑样本的生化、mRNA 表达和组织病理学，评估 D-半乳糖引起的氧化改变以及 EU 和 CAR 的保护作用。结果表明，D-半乳糖导致大脑的氧化改变，这可以通过上调 p53 和 p21 mRNA 表达水平（作为衰老标志物）和 Bax mRNA 表达水平（作为凋亡标志物）来识别。此外，还观察到生化标志物肌酸磷酸激酶（CPK）和三酰甘油（TAG）水平的改变，以及大脑抗氧化能力的增强。最后，将这些结果与用 EU 和 CAR 治疗的组进行比较，观察到 EU 和 CAR 以剂量依赖的方式潜在地减轻这些与衰老相关的氧化改变。最后，可以得出结论，EU 和 CAR 的补充被认为是有前途的天然保护化合物，可以延缓衰老并保持健康。
