Department of Biochemistry, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.
Biochemistry Department, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, Egypt.
Int J Mol Sci. 2020 Jun 18;21(12):4348. doi: 10.3390/ijms21124348.
Aging is an oxidative stress-associated process that progresses with age. Our aim is to delay or attenuate these oxidative alterations and to keep individuals healthy as they age using natural compounds supplementation. Therefore, we conducted the present study to investigate the protective potentials of quercetin against D-galactose (D-gal)-associated oxidative alterations that were induced experimentally in male Wistar rats. Forty-five rats were randomly allocated into five groups of nine rats each. The groups were a control group that was reared on a basal diet and injected subcutaneously with 120 mg D-gal dissolved in physiological saline solution (0.9% NaCl) per kg body weight daily and quercetin-treated groups that received the same basal diet and subcutaneous daily D-gal injections were supplemented orally with 25, 50, and 100 mg of quercetin per kg body weight for 42 days. Pancreatic and renal samples were subjected to histopathological, immunohistochemical, and relative mRNA expression assessments. Aging (, , , and ), apoptotic (, , and caspase-3 protein), proliferative (Ki67 protein), antiapoptotic ( and Bcl2 protein), inflammatory (, , and ), antioxidant (), and functional markers ( and genes and insulin, glucagon, and podocin proteins) were determined to evaluate the oxidative alterations induced by D-gal and the protective role of quercetin. D-gal caused oxidative alterations of the pancreas and kidneys observed via upregulations of aging, apoptotic, and inflammatory markers and downregulated the antiapoptotic, proliferative, antioxidant, and functional markers. Quercetin potentially attenuated these aging-related oxidative alterations in a dose-dependent manner. Finally, we can conclude that quercetin supplementation is considered as a promising natural protective compound that could be used to delay the aging process and to maintain human health.
衰老是一个与氧化应激相关的过程，随着年龄的增长而进展。我们的目的是使用天然化合物补充剂来延缓或减弱这些氧化改变，使个体随着年龄的增长保持健康。因此，我们进行了本研究，以调查槲皮素对 D-半乳糖（D-gal）诱导的雄性 Wistar 大鼠实验性氧化改变的保护潜力。将 45 只大鼠随机分为五组，每组 9 只。对照组在基础饮食中饲养，并每天皮下注射 120mg D-gal 溶解在生理盐水中（0.9%NaCl），每公斤体重；槲皮素处理组接受相同的基础饮食，并每天皮下注射 D-gal ，同时口服补充 25、50 和 100mg/kg 体重的槲皮素，共 42 天。胰腺和肾脏样本进行组织病理学、免疫组织化学和相对 mRNA 表达评估。衰老（、、、和）、凋亡（、、和 caspase-3 蛋白）、增殖（Ki67 蛋白）、抗凋亡（和 Bcl2 蛋白）、炎症（、、和）、抗氧化（）和功能标志物（和基因和胰岛素、胰高血糖素和 podocin 蛋白）被用来评估 D-gal 诱导的氧化改变和槲皮素的保护作用。D-gal 引起胰腺和肾脏的氧化改变，表现为衰老、凋亡和炎症标志物的上调，以及抗凋亡、增殖、抗氧化和功能标志物的下调。槲皮素以剂量依赖的方式潜在地减弱了这些与衰老相关的氧化改变。最后，我们可以得出结论，槲皮素补充被认为是一种有前途的天然保护化合物，可用于延缓衰老过程并维持人类健康。
