Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China.
Viruses. 2022 Apr 29;14(5):930. doi: 10.3390/v14050930.
Long noncoding RNAs (lncRNAs) function as micro regulators to impact gene expression after multiple pathogen infections, which have been largely studied in the last few years. Although lncRNA studies on lower vertebrates have received less attention than those on mammals, current studies suggest that lncRNA plays an important role in the immune response of fish to pathogen infections. Here, we studied the effect of MIR122HG as the host gene of miR-122 and indirectly negatively regulate MAVS-mediated antiviral immune responses in miiuy croaker (). We found that poly(I:C) significantly increases the host MIR122HG expression. The increased MIR122HG expression inhibited the production of the antiviral immune-related genes IFN-1, ISG15 and Viperin upon SCRV treatment. In addition, MIR122HG can act as a pivotally negative regulator involved in the MAVS-mediated NF-κB and IRF3 signaling pathways, which can effectively avoid an excessive immune response. Additionally, we found that MIR122HG can promote the replication of SCRV. Our study provides evidence about the involvement of lncRNAs in the antiviral immune response of fish and broadens the understanding of the function of lncRNAs as a precursor miRNA in teleost fish.
长链非编码 RNA（lncRNAs）作为微调节剂，在多种病原体感染后影响基因表达，这在过去几年中得到了广泛研究。尽管对低等脊椎动物的 lncRNA 研究不如对哺乳动物的研究受到关注，但目前的研究表明，lncRNA 在鱼类对病原体感染的免疫反应中发挥着重要作用。在这里，我们研究了 MIR122HG 作为 miR-122 的宿主基因，并间接负调控鱼类 MAVS 介导的抗病毒免疫反应的作用。我们发现聚（I：C）显著增加了宿主 MIR122HG 的表达。这种增加的 MIR122HG 表达抑制了 SCRV 处理后抗病毒免疫相关基因 IFN-1、ISG15 和 Viperin 的产生。此外，MIR122HG 可以作为一个关键的负调控因子，参与 MAVS 介导的 NF-κB 和 IRF3 信号通路，从而有效避免过度的免疫反应。此外，我们发现 MIR122HG 可以促进 SCRV 的复制。我们的研究提供了证据表明 lncRNAs 参与了鱼类的抗病毒免疫反应，并拓宽了人们对 lncRNA 作为硬骨鱼类前体 miRNA 的功能的理解。
