Rautenberg P, Stender F, Ullmann U
Eur J Clin Microbiol. 1987 Feb;6(1):62-4. doi: 10.1007/BF02097196.
Tests of polyclonal antisera to homogeneous enterotoxin preparations of Clostridium difficile by immunoblotting demonstrated that cross-reactions occurred with proteins from cell lysates of nearly all Clostridium species. There was a notable reduction in the amount of crossreaction when only culture supernatants were analysed. The enterotoxin could be easily identified by its unique high molecular weight of 230,000. The findings suggest that care must be taken when immunological tests other than immunoblotting are used for detection of Clostridium difficile enterotoxin.
通过免疫印迹法对艰难梭菌同源肠毒素制剂的多克隆抗血清进行检测，结果表明，几乎所有梭菌属菌种的细胞裂解液中的蛋白质都发生了交叉反应。当仅分析培养上清液时，交叉反应的量显著减少。肠毒素因其独特的230,000高分子量而易于识别。研究结果表明，在使用免疫印迹法以外的免疫检测方法检测艰难梭菌肠毒素时必须谨慎。
