射血分数降低的心力衰竭患者循环内皮细胞和内皮祖细胞水平降低。

Reduced Levels of Circulating Endothelial Cells and Endothelial Progenitor Cells in Patients with Heart Failure with Reduced Ejection Fraction.

作者信息

Lopes José, Teixeira Manuel, Cavalcante Suiane, Gouveia Marisol, Duarte Ana, Ferreira Miriam, Simões Maria I, Conceição Maria, Ribeiro Ilda P, Gonçalves Ana C, Schmidt Cristine, de Jesus Bruno Bernardes, Almeida Ramiro, Viamonte Sofia, Santos Mário, Ribeiro Fernando

机构信息

Institute of Biomedicine-iBiMED, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal.

Research Centre in Physical Activity, Health and Leisure, CIAFEL, Faculty of Sport, University of Porto, Porto, Portugal.

出版信息

Arch Med Res. 2022 Apr;53(3):289-295. doi: 10.1016/j.arcmed.2022.02.001. Epub 2022 Feb 17.

DOI:10.1016/j.arcmed.2022.02.001

PMID:35183379

Abstract

BACKGROUND

Endothelial dysfunction has been suggested as a potential mechanism contributing to the development and progression of heart failure (HF). Levels of circulating endothelial cells (CECs), endothelial progenitor cells (EPCs), and hematopoietic stem and progenitor cells (HSPCs) have been recognized as useful markers of vascular damage and endothelial repair in response to tissue injury.

AIMS

To evaluate the circulating levels of EPCs, CECs, and HSPCs among patients with HF with reduced ejection fraction (HFrEF).

METHODS

In 82 individuals (42 patients with HFrEF and 42 age-matched subjects without established cardiovascular disease), peripheral blood was drawn and levels of EPCs, CECs, and HSPCs were quantified by flow cytometry.

RESULTS

Patients with HFrEF showed lower levels of circulating EPCs (5.28 × 10 ± 6.83 × 10% vs. 7.76 × 10 ± 4.91 × 10%, p ≤0.001) and CECs (5.11 × 10 ± 7.87 × 10% vs. 6.51 × 10 ± 5.21 × 10%, p = 0.005) when compared to the age-matched group. Circulating levels of HSPCs were not significantly different between groups (p = 0.590). Additionally, the number of EPCs and CECs was significantly higher in HFrEF patients with overweight/obesity (n = 24) compared to patients with normal weight (n = 17).

CONCLUSION

Circulating levels of EPCs and CECs were significantly decreased in patients with HFrEF in comparison to age-matched subjects without established cardiovascular disease, suggesting that the levels of CECs and EPCs may be potential biomarkers of the cellular response to vascular injury in patients with HFrEF.

摘要

背景

内皮功能障碍被认为是导致心力衰竭（HF）发生和发展的潜在机制。循环内皮细胞（CEC）、内皮祖细胞（EPC）以及造血干细胞和祖细胞（HSPC）的水平已被视为血管损伤和内皮修复对组织损伤反应的有用标志物。

目的

评估射血分数降低的心力衰竭（HFrEF）患者中EPC、CEC和HSPC的循环水平。

方法

在82名个体（42例HFrEF患者和42名年龄匹配且无心血管疾病的受试者）中，采集外周血，通过流式细胞术对EPC、CEC和HSPC的水平进行定量。

结果

与年龄匹配组相比，HFrEF患者的循环EPC水平较低（5.28×10±6.83×10%对7.76×10±4.91×10%，p≤0.001），CEC水平也较低（5.11×10±7.87×10%对6.51×10±5.21×10%，p = 0.005）。两组之间HSPC的循环水平无显著差异（p = 0.590）。此外，超重/肥胖的HFrEF患者（n = 24）的EPC和CEC数量明显高于体重正常的患者（n = 17）。