Tao Hongzheng, Jia Zhiqiang, Gao Xue, Gui Min, Li Yongzhong, Liu Yating
College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; College of Biological and Agricultural Sciences, Honghe University, Mengzi 661199, China.
College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
Virus Res. 2022 Apr 15;312:198710. doi: 10.1016/j.virusres.2022.198710. Epub 2022 Feb 18.
MicroRNAs (miRNAs) are a class of about 22 nucleotide endogenous non-coding small RNAs that play important roles in plant development and defense responses to biotic and abiotic stress. Tomato spotted wilt orthotospovirus (TSWV) is among the most devastating plant viruses worldwide, causing leaf spotting and wilting symptoms in many plants. In this study, high-throughput sequencing technology was used to identify miRNAs in peppers that were regulated by TSWV infection, and bioinformatics techniques were used to analyze the function of these miRNAs and their target genes. The sequencing results showed that, in the pepper plants infected by TSWV, a total of 101 miRNAs were differentially expressed, and several differentially expressed miRNAs were verified by the qRT-PCR method. Based on the GO function and KEGG pathway enrichment analysis, the functions of these miRNAs and their target genes were predicted. These target genes play a regulatory role in pathways such as plant-pathogen interactions and plant hormone signal transduction. Our results provide information about pepper miRNAs and will help to further understand the mechanism of TSWV-host interactions.
微小RNA（miRNA）是一类约22个核苷酸的内源性非编码小RNA，在植物发育以及对生物和非生物胁迫的防御反应中发挥重要作用。番茄斑萎正黏病毒（TSWV）是全球最具毁灭性的植物病毒之一，可在许多植物中引发叶片斑点和萎蔫症状。在本研究中，利用高通量测序技术鉴定受TSWV感染调控的辣椒miRNA，并运用生物信息学技术分析这些miRNA及其靶基因的功能。测序结果表明，在受TSWV感染的辣椒植株中，共有101个miRNA差异表达，并且通过qRT-PCR方法验证了几个差异表达的miRNA。基于基因本体（GO）功能和京都基因与基因组百科全书（KEGG）通路富集分析，预测了这些miRNA及其靶基因的功能。这些靶基因在植物-病原体相互作用和植物激素信号转导等通路中发挥调控作用。我们的结果提供了有关辣椒miRNA的信息，并将有助于进一步了解TSWV-寄主相互作用的机制。
