College of Plant Protection, Northwest Agriculture and Forestry University, Xianyang 712100, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Int J Mol Sci. 2023 Feb 12;24(4):3675. doi: 10.3390/ijms24043675.
MicroRNAs play key regulatory roles in plant development. The changed pattern of miRNA expression is involved in the production of viral symptoms. Here, we showed that a small RNA, Seq119, a putative novel microRNA, is associated with the low seed setting rate, a viral symptom of rice stripe virus (RSV)-infected rice. The expression of Seq 119 was downregulated in RSV-infected rice. The overexpression of Seq119 in transgenic rice plants did not cause any obvious phenotypic changes in plant development. When the expression of Seq119 was suppressed in rice plants either by expressing a mimic target or by CRISPR/Cas editing, seed setting rates were extremely low, similar to the effects of RSV infection. The putative targets of Seq119 were then predicted. The overexpression of the target of Seq119 in rice caused a low seed setting rate, similar to that in Seq119-suppressed or edited rice plants. Consistently, the expression of the target was upregulated in Seq119-suppressed and edited rice plants. These results suggest that downregulated Seq119 is associated with the low seed setting rate symptom of the RSV in rice.
MicroRNAs 在植物发育中发挥关键的调节作用。miRNA 表达模式的改变与病毒症状的产生有关。在这里，我们发现一个小 RNA Seq119，一种假定的新型 microRNA，与水稻条纹病毒（RSV）感染水稻的低结实率这一病毒症状有关。Seq119 的表达在 RSV 感染的水稻中下调。Seq119 在转基因水稻植株中的过表达并未导致植物发育出现任何明显的表型变化。当通过表达模拟物靶标或 CRISPR/Cas 编辑来抑制水稻中 Seq119 的表达时，结实率极低，类似于 RSV 感染的效果。然后预测了 Seq119 的假定靶标。在水稻中过表达 Seq119 的靶标导致结实率低，类似于 Seq119 抑制或编辑的水稻植株。一致地，靶标的表达在 Seq119 抑制和编辑的水稻植株中上调。这些结果表明，下调的 Seq119 与 RSV 感染水稻中的低结实率症状有关。
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