Ou Yichao, Che Mengjie, Peng Junjie, Zhou Mingfeng, Wu Guangsen, Gong Haodong, Li Kai, Wang Xingqin, Niu Peirong, Qi Songtao, Feng Zhanpeng
Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
The Laboratory for Precision Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Front Neuroanat. 2022 Feb 4;16:711138. doi: 10.3389/fnana.2022.711138. eCollection 2022.
The hypothalamus is the key region that regulates the neuroendocrine system as well as instinct behaviors, and hypothalamic dysfunction causes refractory clinical problems. Recent studies have indicated that neural stem/progenitor cell (NSPC) in the hypothalamus play a crucial role in hypothalamic function. However, specific hypothalamic NSPC culture methods have not been established, especially not detailed or efficient surgical procedures. The present study presented a convenient, detailed and efficient method for the isolation and cultivation of hypothalamic NSPCs from embryonic day 12.5 mice. The procedure includes embryo acquisition, brain microdissection to quickly obtain hypothalamic tissue and hypothalamic NSPC culture. Hypothalamic NSPCs can be quickly harvested and grow well in both neurosphere and adherent cultures through this method. Additionally, we confirmed the cell origin and evaluated the proliferation and differentiation properties of cultured cells. In conclusion, we present a convenient and practical method for the isolation and cultivation of hypothalamic NSPCs that can be used in extensive hypothalamic studies.
下丘脑是调节神经内分泌系统以及本能行为的关键区域，下丘脑功能障碍会引发难治性临床问题。最近的研究表明，下丘脑的神经干/祖细胞（NSPC）在维持下丘脑功能方面发挥着关键作用。然而，尚未建立特定的下丘脑NSPC培养方法，尤其是缺乏详细且高效的手术操作流程。本研究提出了一种简便、详细且高效的方法，用于从胚胎第12.5天的小鼠中分离和培养下丘脑NSPC。该操作包括获取胚胎、进行脑显微解剖以快速获得下丘脑组织以及培养下丘脑NSPC。通过这种方法，下丘脑NSPC能够被快速收获，并且在神经球培养和贴壁培养中均能良好生长。此外，我们还确定了细胞来源，并评估了培养细胞的增殖和分化特性。总之，我们提出了一种简便实用的下丘脑NSPC分离和培养方法，可用于广泛的下丘脑研究。
