Zhu Xiaoping, Xu Tianyi, Peng Chen, Wu Shihua
Joint Research Centre for Engineering Biology, Zhejiang University-University of Edinburgh Institute, Zhejiang University, Haining, China.
Research Center of Siyuan Natural Pharmacy and Biotoxicology, College of Life Sciences, Zhejiang University, Hangzhou, China.
Front Chem. 2022 Feb 3;9:782432. doi: 10.3389/fchem.2021.782432. eCollection 2021.
Compared with conventional optical microscopy techniques, mass spectrometry imaging (MSI) or imaging mass spectrometry (IMS) is a powerful, label-free analytical technique, which can sensitively and simultaneously detect, quantify, and map hundreds of biomolecules, such as peptides, proteins, lipid, and other organic compounds in cells and tissues. So far, although several soft ionization techniques, such as desorption electrospray ionization (DESI) and secondary ion mass spectrometry (SIMS) have been used for imaging biomolecules, matrix-assisted laser desorption/ionization (MALDI) is still the most widespread MSI scanning method. Here, we aim to provide a comprehensive review of MALDI-MSI with an emphasis on its advances of the instrumentation, methods, application, and future directions in single cell and biological tissues.
与传统光学显微镜技术相比，质谱成像（MSI）或成像质谱（IMS）是一种强大的、无需标记的分析技术，它能够灵敏且同时检测、定量并绘制细胞和组织中数百种生物分子，如肽、蛋白质、脂质及其他有机化合物。到目前为止，尽管已经有几种软电离技术，如解吸电喷雾电离（DESI）和二次离子质谱（SIMS）被用于生物分子成像，但基质辅助激光解吸/电离（MALDI）仍然是最广泛应用的MSI扫描方法。在此，我们旨在对MALDI-MSI进行全面综述，重点关注其在仪器、方法、应用以及单细胞和生物组织中的未来发展方向方面的进展。
