Davis Rebecca J, Belikoff Esther J, Dickey Allison N, Scholl Elizabeth H, Benoit Joshua B, Scott Maxwell J
Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC 27695-7613, USA.
Bioinformatics Research Center, North Carolina State University, Campus Box 7566, Raleigh, NC 27695-7566, USA.
Genomics. 2021 Nov;113(6):3978-3988. doi: 10.1016/j.ygeno.2021.10.003. Epub 2021 Oct 5.
The common green bottle blow fly Lucilia sericata (family, Calliphoridae) is widely used for maggot debridement therapy, which involves the application of sterile maggots to wounds. The larval excretions and secretions are important for consuming necrotic tissue and inhibiting bacterial growth in wounds of patients. Lucilia sericata is also of importance as a pest of sheep and in forensic studies to estimate a postmortem interval. Here we report the assembly of a 565.3 Mb genome from long read PacBio DNA sequencing of genomic DNA. The genome contains 14,704 predicted protein coding genes and 1709 non-coding genes. Targeted annotation and transcriptional analyses identified genes that are highly expressed in the larval salivary glands (secretions) and Malpighian tubules (excretions) under normal growth conditions and following heat stress. The genomic resources will underpin future genetic studies and in development of engineered strains for genetic control of L. sericata and for biotechnology-enhanced maggot therapy.
常见的绿头苍蝇丝光绿蝇（丽蝇科）被广泛用于蛆虫清创疗法，该疗法涉及将无菌蛆虫应用于伤口。幼虫的排泄物和分泌物对于消耗坏死组织以及抑制患者伤口中的细菌生长非常重要。丝光绿蝇作为绵羊害虫以及在法医研究中用于估计死后间隔时间也具有重要意义。在此，我们报告了通过对基因组DNA进行长读长PacBio DNA测序组装得到的565.3 Mb基因组。该基因组包含14704个预测的蛋白质编码基因和1709个非编码基因。靶向注释和转录分析确定了在正常生长条件下以及热应激后在幼虫唾液腺（分泌物）和马氏管（排泄物）中高表达的基因。这些基因组资源将为未来的遗传研究以及开发用于丝光绿蝇基因控制和生物技术增强蛆虫疗法的工程菌株奠定基础。
