Yeong Y S, Nazni W A, Santana R L, Mohd Noor I, Lee H L, Mohd Sofian A
Medical Entomology Unit, Infectious Diseases Research Centre (IDRC), Institute for Medical Research, Kuala Lumpur, Malaysia.
Trop Biomed. 2011 Aug;28(2):325-32.
In Malaysia, maggot debridement therapy (MDT) utilizes maggots of Lucilia cuprina (Wiedemann) to debride necrotic tissue from wound surface, reduce bacterial infection and therefore, enhance wound healing process. To evaluate the sterility of the sterile maggots produced after sterilization process before delivering onto patient wounds. Sterility of sterile maggots is crucial in ensuring the safe usage of MDT and patient's health. Eggs of L. cuprina collected from a laboratory colony were divided into treated group (sterilized) and control group (non-sterilized). Treated group underwent sterilization while eggs from control group were allowed to hatch without sterilization. Sodium hypochlorite and formaldehyde were the main disinfectants used in this sterilization process. Scanning electron microscope (SEM) was used to examine and ascertain the sterility of sterile maggots. SEM results showed that all sterilized L. cuprina eggs and maggots achieved sterility and all were cleared from bacterial contamination. In contrast, all non-sterilized eggs and maggots were found to be colonized by microorganisms. Sterilization method employed to sterilize eggs and maggots used in Malaysia MDT was proven successful and MDT is safe to be used as wound management tools.
在马来西亚，蛆虫清创疗法（MDT）利用铜绿蝇（维德曼）的蛆虫清除创面坏死组织，减少细菌感染，从而加速伤口愈合过程。在将灭菌后的无菌蛆虫应用于患者伤口之前，需评估其无菌状态。无菌蛆虫的无菌性对于确保MDT的安全使用及患者健康至关重要。从实验室种群收集的铜绿蝇卵被分为处理组（灭菌）和对照组（未灭菌）。处理组进行灭菌处理，而对照组的卵未经灭菌直接孵化。次氯酸钠和甲醛是该灭菌过程中使用的主要消毒剂。使用扫描电子显微镜（SEM）检查并确定无菌蛆虫的无菌状态。SEM结果显示，所有经灭菌的铜绿蝇卵和蛆虫均达到无菌状态，且所有细菌污染均已清除。相比之下，所有未灭菌的卵和蛆虫均被微生物定殖。马来西亚MDT中用于卵和蛆虫灭菌的方法被证明是成功的，MDT作为伤口处理工具使用是安全的。
