Patel B T, Tudball N
Brain Res. 1986 Apr 9;370(2):250-64. doi: 10.1016/0006-8993(86)90480-4.
Antisera against rat-liver S-adenosylhomocysteine hydrolase (SAH-hydrolase) and calf intestinal mucosal adenosine deaminase (ADA) were raised in rabbits and subsequently used to determine the distribution of the corresponding enzymes in rat-brain using the peroxidase-antiperoxidase immunohistochemical procedure. SAH-hydrolase antigenicity was prominent in the neocortex, hippocampal formation, cerebellum and olfactory tubercle. In the cerebellum, only those cells associated with the Purkinje layer possessed pronounced reactivity with anti-SAH hydrolase. The intense staining present could be correlated mainly with nuclei, the cytosol being stained less intensely. Weak ADA antigenicity was found throughout the brain, but strong antigenic reactivity was associated with neurones in the basal hypothalamus, superior colliculus and in nerve fibres in many regions. Many ADA antigenic neurones and fibres were seen in close proximity to blood vessels. The distribution of ADA antigenicity was also studied in cat and rabbit brain. In cat brain only general staining of tissue occurred with anti-ADA and no intensely stained regions comparable to those seen in rat brain were observed. Rabbit brain showed weak specifically stained neurones only in the superior colliculus. Enzyme assays were also performed to confirm immunohistochemical findings. There appears to be little in common between regions which stained intensely with anti-SAH hydrolase and anti-ADA respectively. The possible implications of these findings are discussed.
用兔制备了抗大鼠肝脏S-腺苷同型半胱氨酸水解酶（SAH水解酶）和小牛肠黏膜腺苷脱氨酶（ADA）的抗血清，随后使用过氧化物酶-抗过氧化物酶免疫组织化学方法来测定相应酶在大鼠脑中的分布。SAH水解酶的抗原性在新皮层、海马结构、小脑和嗅结节中很突出。在小脑中，只有那些与浦肯野层相关的细胞与抗SAH水解酶有明显的反应性。呈现的强烈染色主要与细胞核相关，细胞质染色较弱。在整个大脑中都发现了较弱的ADA抗原性，但在基底下丘脑、上丘以及许多区域的神经纤维中，强抗原反应性与神经元相关。在许多靠近血管的地方可以看到许多具有ADA抗原性的神经元和纤维。还研究了ADA抗原性在猫和兔脑中的分布。在猫脑中，抗ADA只对组织进行了一般染色，未观察到与大鼠脑中所见类似的强染色区域。兔脑仅在上丘显示出弱阳性的特异性染色神经元。还进行了酶活性测定以证实免疫组织化学的结果。分别用抗SAH水解酶和抗ADA强烈染色的区域之间似乎没有什么共同之处。讨论了这些发现可能的意义。
