Senba E, Daddona P E, Nagy J I
Brain Res Bull. 1986 Aug;17(2):209-17. doi: 10.1016/0361-9230(86)90117-6.
Immunohistochemical procedures were used to determine the localization of adenosine deaminase (ADA) in the rat retina. Small ADA-immunoreactive neurons having a sparse but regular distribution pattern were detected in the ganglion cell layer (GCL) and in somewhat fewer numbers in the inner nuclear layer (INL). ADA-immunoreactive processes eminating from these two cell types were seen distributed in specific sublayers of the inner plexiform layer (IPL). In addition, a dense band of punctate ADA-immunostaining was observed in the IPL immediately adjacent to the GCL. Injections of the retrogradely transported dye, fast blue, into the optic nerve failed to label ADA-immunoreactive neurons in the GCL and unilateral enucleation had no effect on the density of ADA-immunostained fibers in the superior colliculus or lateral geniculate nucleus on the enucleated compared with the contralateral control side. In addition, ADA-immunoreactive cells in the GCL of the rat appeared not to correspond to the population of cells in this layer which in other species have been shown to accumulate 4,6-diamino-2-phenylindole (DAPI) following intraocular injection of this dye. These results indicate that subpopulations of intrinsic neurons in the rat retina express high levels of ADA.
采用免疫组织化学方法确定大鼠视网膜中腺苷脱氨酶（ADA）的定位。在神经节细胞层（GCL）中检测到具有稀疏但规则分布模式的小的ADA免疫反应性神经元，在内核层（INL）中的数量略少。源自这两种细胞类型的ADA免疫反应性突起分布在内网状层（IPL）的特定亚层中。此外，在紧邻GCL的IPL中观察到一条密集的点状ADA免疫染色带。将逆行运输染料快蓝注入视神经未能标记GCL中的ADA免疫反应性神经元，与对侧对照侧相比，单侧眼球摘除对摘除侧上丘或外侧膝状体中ADA免疫染色纤维的密度没有影响。此外，大鼠GCL中的ADA免疫反应性细胞似乎与该层中在眼内注射这种染料后已显示积累4,6-二氨基-2-苯基吲哚（DAPI）的细胞群体不一致。这些结果表明，大鼠视网膜中固有神经元的亚群表达高水平的ADA。
