Durrant L G, Robins R A, Armitage N C, Brown A, Baldwin R W, Hardcastle J D
Cancer Res. 1986 Jul;46(7):3543-9.
Fifty colorectal tumors were screened by indirect immunofluorescence and flow cytometry for antigen expression using a panel of monoclonal antibodies that recognize determinants preferentially expressed on tumor cells (carcinoembryonic antigen, Y haptenic blood group, 791T/36 defined antigen 791T-P72). Fifty % of the tumors expressed all three antigens, 41%, two, and 9%, one. Over a third reacted strongly with at least one monoclonal antibody, although the majority of tumors stained with a moderate intensity. Extranuclear membranes from tumors showed similar antigen expression to disaggregated tumor cells and were particularly useful for providing the relative tumor:normal tissue binding ratios. The carcinoembryonic antigen specific monoclonal antibody showed the strongest tumor selectivity with a tumor:normal tissue ratio of 24 +/- 7:1. Lack of correlation between expression of the three antigens suggested that the monoclonal antibodies recognizing them may have potential as a "cocktail." One-third of the tumors contained cells with an aneuploid DNA content and expressed elevated levels of carcinoembryonic antigen and Y haptenic blood group antigen when compared to tumors with diploid DNA content. Aneuploid cells within a tumor were also preferentially stained with all of the monoclonal antibodies.
使用一组识别优先在肿瘤细胞上表达的决定簇的单克隆抗体（癌胚抗原、Y 血型抗原、791T/36 定义抗原 791T-P72），通过间接免疫荧光和流式细胞术对 50 个结直肠肿瘤进行抗原表达筛查。50%的肿瘤表达所有三种抗原，41%表达两种，9%表达一种。超过三分之一的肿瘤与至少一种单克隆抗体强烈反应，尽管大多数肿瘤染色强度适中。肿瘤的核外膜显示出与解离的肿瘤细胞相似的抗原表达，并且对于提供相对肿瘤：正常组织结合比率特别有用。癌胚抗原特异性单克隆抗体显示出最强的肿瘤选择性，肿瘤：正常组织比率为 24±7:1。三种抗原表达之间缺乏相关性表明，识别它们的单克隆抗体可能有作为“鸡尾酒”的潜力。与具有二倍体 DNA 含量的肿瘤相比，三分之一的肿瘤含有非整倍体 DNA 含量的细胞，并且癌胚抗原和 Y 血型抗原水平升高。肿瘤内的非整倍体细胞也优先被所有单克隆抗体染色。
