对临床产OXA-48碳青霉烯耐药粘质沙雷氏菌菌株进行全基因组、耐药组和毒力组综合分析。

Comprehensive pan-genomic, resistome and virulome analysis of clinical OXA-48 producing carbapenem-resistant Serratia marcescens strains.

作者信息

Bolourchi Negin, Noori Goodarzi Narjes, Giske Christian G, Nematzadeh Shoeib, Haririzadeh Jouriani Fatemeh, Solgi Hamid, Badmasti Farzad

机构信息

Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Gene. 2022 May 15;822:146355. doi: 10.1016/j.gene.2022.146355. Epub 2022 Feb 18.

DOI:10.1016/j.gene.2022.146355

PMID:35189248

Abstract

BACKGROUND

Carbapenem-resistant Enterobacteriaceae (CRE) have been thoroughly studied as the pathogens associated with hospital acquired infections. However, data on Serratia marcescens are not enough. S. marcescens is now becoming a propensity for its highly antimicrobial-resistant clinical infections.

METHODS

Four carbapenem-resistant S. marcescens (CR-SM) isolates were obtained from hospitalized patients through routine microbiological experiments. We assembled the isolates genomes using whole genome sequencing (WGS) and compared their resistome and virulome patterns.

RESULTS

The average length and CG content of chromosomes was 5.33 Mbp and 59.8%, respectively. The number of coding sequences (CDSs) ranged from 4,959 to 4,989. All strains had one single putative conjugative plasmid with IncL incompatibility (Inc) group. The strains harbored bla, bla and bla. All plamsids were positive for bla. No bla, bla, bla and bla were identified. The bla and aac(6')-Ic genes were chromosomally-encoded. Class 1 integron was detected in strains P8, P11 and P14. The Escher_RCS47 and Salmon_SJ46 prophages played major role in plasmid-mediated carraige of extended spectrum β-lactamases (ESBLs). The CR-SM strains were equipt with typical virulence factors of oppotunistic pathogens including biofilm formation, adhesins, secretory systems and siderophores. The strains did not have ability to produce prodigiosin but were positive for chitinase and EstA.

CONCLUSION

The presence of conjugative plasmids harboring major β-lactamases within prophage and class 1 integron structures highlights the role of different mobile genetic elements (MGEs) in distribution of AMR factors and more specifically carbapenemases. More molecular studies are required to determine the status of carbapenem resistance in clinical starins. However, appropriate strategies to control the global dissemination of CR-SM are urgent.

摘要

背景

耐碳青霉烯类肠杆菌科细菌（CRE）作为与医院获得性感染相关的病原体已得到充分研究。然而，关于粘质沙雷氏菌的数据还不够。粘质沙雷氏菌因其高度耐药的临床感染正日益受到关注。

方法

通过常规微生物学实验从住院患者中获得4株耐碳青霉烯类粘质沙雷氏菌（CR-SM）分离株。我们使用全基因组测序（WGS）组装分离株基因组，并比较它们的耐药基因组和毒力基因组模式。

结果

染色体的平均长度和CG含量分别为5.33 Mbp和59.8%。编码序列（CDS）的数量在4959至4989之间。所有菌株都有一个单一的假定接合质粒，属于IncL不相容（Inc）群。这些菌株携带bla、bla和bla。所有质粒bla均为阳性。未鉴定到bla、bla、bla和bla。bla和aac(6')-Ic基因是染色体编码的。在菌株P8、P11和P14中检测到1类整合子。Escher_RCS47和Salmon_SJ46原噬菌体在质粒介导的超广谱β-内酰胺酶（ESBLs）携带中起主要作用。CR-SM菌株具备机会性病原体的典型毒力因子，包括生物膜形成、粘附素、分泌系统和铁载体。这些菌株没有产生灵菌红素的能力，但几丁质酶和EstA呈阳性。