Salisbury J L, Baron A T, Coling D E, Martindale V E, Sanders M A
Cell Motil Cytoskeleton. 1986;6(2):193-7. doi: 10.1002/cm.970060218.
Affinity-purified antibodies that recognize the 20,000-dalton molecular weight (20 kd) striated flagellar root protein of Tetraselmis striata have been used to identify antigenic homologs in other eucaryotic organisms of diverse evolutionary origins. Among the green algae, Tetraselmis and Chlamydomonas, and their colorless relative, Polytomella, the 20-kd homologs appear associated with basal bodies. This occurs most prominently in the form of flagellar roots of both striated and microtubule subtended types. Among cultured mammalian cells (PtK2 and primary mouse macrophage cell lines), flagellar root protein homologs appear as basal feet, pericentriolar fibrils, and pericentriolar satellites. Mammalian sperm cells also show flagellar root protein homologs associated with their basal bodies. We envisage a functional role for these fibrous calcium-sensitive contractile proteins in altering the orientation of centrioles or basal bodies with their associated MTOCs by responding to topological calcium fluxes.
已使用能识别条纹鞭毛藻（Tetraselmis striata）20,000道尔顿分子量（20 kd）条纹鞭毛根蛋白的亲和纯化抗体，来鉴定其他具有不同进化起源的真核生物中的抗原同源物。在绿藻、条纹鞭毛藻和衣藻以及它们无色的亲缘物种多鞭藻中，20-kd同源物似乎与基体相关。这在条纹型和微管支撑型鞭毛根中最为明显。在培养的哺乳动物细胞（PtK₂和原代小鼠巨噬细胞系）中，鞭毛根蛋白同源物表现为基足、中心粒周围纤维和中心粒周围卫星体。哺乳动物精子细胞也显示鞭毛根蛋白同源物与其基体相关。我们设想这些纤维状钙敏感收缩蛋白通过响应拓扑钙通量，在改变中心粒或基体及其相关微管组织中心的方向方面发挥功能作用。
