European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
Institute of Biological and Chemical Systems, Biological Information Processing (IBCS-BIP), Karlsruhe Institute of Technology, 76131, Karlsruhe, Germany.
Genome Biol. 2022 Feb 21;23(1):59. doi: 10.1186/s13059-022-02623-z.
Unraveling the relationship between genetic variation and phenotypic traits remains a fundamental challenge in biology. Mapping variants underlying complex traits while controlling for confounding environmental factors is often problematic. To address this, we establish a vertebrate genetic resource specifically to allow for robust genotype-to-phenotype investigations. The teleost medaka (Oryzias latipes) is an established genetic model system with a long history of genetic research and a high tolerance to inbreeding from the wild.
Here we present the Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel: the first near-isogenic panel of 80 inbred lines in a vertebrate model derived from a wild founder population. Inbred lines provide fixed genomes that are a prerequisite for the replication of studies, studies which vary both the genetics and environment in a controlled manner, and functional testing. The MIKK panel will therefore enable phenotype-to-genotype association studies of complex genetic traits while allowing for careful control of interacting factors, with numerous applications in genetic research, human health, drug development, and fundamental biology.
Here we present a detailed characterization of the genetic variation across the MIKK panel, which provides a rich and unique genetic resource to the community by enabling large-scale experiments for mapping complex traits.
揭示遗传变异与表型特征之间的关系仍然是生物学中的一个基本挑战。在控制混杂环境因素的情况下，对复杂性状的潜在变异进行映射通常是有问题的。为了解决这个问题，我们专门建立了一个脊椎动物遗传资源库，以允许进行强大的基因型-表型研究。硬骨鱼中的模式生物——青鳉（Oryzias latipes）是一个经过长期遗传研究并具有较高的野生近交耐受能力的遗传模型系统。
在这里，我们介绍了近交系青鳉基因库（MIKK）面板：这是第一个从野生种群中衍生出来的脊椎动物模型的近交系 80 个品系的近交系面板。近交系提供了固定的基因组，这是研究复制的前提，这些研究以受控的方式改变遗传和环境，并进行功能测试。因此，MIKK 面板将能够对复杂遗传性状进行表型-基因型关联研究，同时允许仔细控制相互作用的因素，在遗传研究、人类健康、药物开发和基础生物学等领域具有广泛的应用。
在这里，我们对 MIKK 面板的遗传变异进行了详细的描述，该面板为社区提供了丰富而独特的遗传资源，通过进行大规模的复杂性状作图实验。
