School of Chemistry, Xi'an Jiaotong University, Xi'an, 710049, China.
School of Chemistry, Xi'an Jiaotong University, Xi'an, 710049, China.
Talanta. 2021 Dec 1;235:122798. doi: 10.1016/j.talanta.2021.122798. Epub 2021 Aug 12.
Covalent organic frameworks (COFs) are considered to be a promising support material for catalyst due to their highly ordered porous structure. Here, a core-shell structured FeO magnetic covalent organic framework (FeO@COF) was synthesized and employed to provide basic sites for immobilization of gold nanoparticles (AuNPs). The AuNPs was in-situ immobilized on the shell of FeO@COF via a citrate reducing method. The FeO@COF-AuNP had convenient magnetic separability and exhibited excellent mimicking peroxidase-like activity in catalyzing chemiluminescence (CL) reaction of luminol with hydrogen peroxide (HO). With acetylcholine chloride (ACh) as substrate of acetylcholinesterase (AChE), a CL method was exploited for sensitive detection of organophosphorus pesticide triazophos due to its irreversible inhibiting effect on the AChE activity and subsequently influences the production of HO under the condition of choline oxidase (ChOx). This method gave a good linearity for triazophos in the range of 5.0-300.0 nmol L, and a limit of detection (LOD) of 1 nmol L was acquired. The applicability of this method was verified by the determination of triazophos in different spiked vegetable samples.
共价有机骨架（COFs）因其具有高度有序的多孔结构而被认为是一种有前途的催化剂载体材料。在此，合成了核壳结构的 FeO 磁性共价有机骨架（FeO@COF），并将其用于提供碱性位以固定金纳米粒子（AuNPs）。通过柠檬酸还原法将 AuNPs 原位固定在 FeO@COF 的壳上。FeO@COF-AuNP 具有方便的磁分离性能，并在催化鲁米诺与过氧化氢（HO）的化学发光（CL）反应中表现出优异的类过氧化物酶活性。以乙酰胆碱氯化物（ACh）作为乙酰胆碱酯酶（AChE）的底物，由于其对 AChE 活性的不可逆抑制作用以及随后在胆碱氧化酶（ChOx）条件下对 HO 生成的影响，CL 法可用于灵敏检测有机磷农药三唑磷。该方法在 5.0-300.0 nmol L 的范围内对三唑磷具有良好的线性，检测限（LOD）为 1 nmol L。该方法通过测定不同蔬菜样品中的三唑磷验证了其适用性。
