Laboratório de Virologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Laboratório de Citometria de Fluxo, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
J Neurovirol. 2022 Apr;28(2):291-304. doi: 10.1007/s13365-022-01054-7. Epub 2022 Feb 21.
HIV-1 subtype C (HIV-1C) shows reduced Tat protein chemoattractant activity compared with HIV-1B. The impact of HIV-1C Tat on the chemotaxis of the main lymphocyte subpopulations in the cerebrospinal fluid (CSF) and the peripheral blood (PB) is unclear. We hypothesized that there would be a lower frequency of specific lymphocyte subpopulations CD3 or CD19 in CSF in HIV-1C than in HIV-1B. The objectives were to detect the differences in the proportions of main lymphocyte subpopulations in CSF and PB, between people with HIV (PWH) and HIV-1-uninfected volunteers (PWoH) and in HIV-1B and HIV-1C. Lymphocyte immunophenotyping was studied in CSF and paired PB samples of PWH (n = 22) and PWoH (n = 14). Lymphocytes were analyzed within the CD45 gated region. The proportions of CSF CD3CD4, CD3CD8, and CD3CD19 lymphocytes in CSF were comparable in HIV-1B and C. There was an increase in the proportion of CD3CD8 cells and a decrease in CD3CD4 T cells (ps = 0.016) in the CSF samples of the PWH compared with the PWoH group. In the PWH group, both CD3CD4 and CD3CD8 lymphocytes were significantly higher in the CSF than in the PB (p = 0.047 and 0.005). The proportion of CD3CD4 was lower and that of CD3CD8 was higher in the CSF samples of the aviremic group than that of HIV-negative control (p = 0.0008 and < 0.0001, respectively). HIV-1C Tat substitution (C30S) did not interfere with the CNS migration of the main lymphocyte subpopulations. This is the first study to evaluate these lymphocytes in CSF and PB of HIV-1C compared with HIV-1B.
HIV-1 亚型 C（HIV-1C）与 HIV-1B 相比，Tat 蛋白的趋化活性降低。HIV-1C Tat 对脑脊液（CSF）和外周血（PB）中主要淋巴细胞亚群趋化作用的影响尚不清楚。我们假设 HIV-1C 中的 CSF 中特定淋巴细胞亚群 CD3 或 CD19 的频率会低于 HIV-1B。目的是检测 HIV-1C 与 HIV-1B 相比，HIV-1 感染者（PWH）和 HIV-1 未感染者（PWoH）CSF 和 PB 中主要淋巴细胞亚群比例的差异。对 22 名 PWH 和 14 名 PWoH 的 CSF 和配对 PB 样本进行了淋巴细胞免疫表型研究。在 CD45 门内分析淋巴细胞。CSF 中 CD3CD4、CD3CD8 和 CD3CD19 淋巴细胞的比例在 HIV-1B 和 C 中是可比的。与 PWoH 组相比，PWH 组 CSF 中 CD3CD8 细胞的比例增加，CD3CD4 T 细胞的比例减少（p = 0.016）。在 PWH 组中，CSF 中 CD3CD4 和 CD3CD8 淋巴细胞均明显高于 PB（p = 0.047 和 0.005）。无病毒血症组 CSF 样本中 CD3CD4 的比例较低，CD3CD8 的比例较高，与 HIV 阴性对照组相比差异有统计学意义（p = 0.0008 和 < 0.0001）。HIV-1C Tat 取代（C30S）并没有干扰主要淋巴细胞亚群向中枢神经系统的迁移。这是第一项比较 HIV-1C 与 HIV-1B 评估这些淋巴细胞在 CSF 和 PB 中的研究。
