Okamoto Wataru, Hasegawa Mai, Usui Tomone, Kashima Tomonori, Sakata Sho, Hamano Tatsuhiko, Onozawa Hiroto, Hashimoto Ryo, Iwazaki Masayuki, Kohno Mitsutomo, Komatsu Teruyuki
Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Tokyo, Japan.
Department of General Thoracic Surgery, School of Medicine, Tokai University, Kanagawa, Japan.
J Biomed Mater Res B Appl Biomater. 2022 Aug;110(8):1827-1838. doi: 10.1002/jbm.b.35040. Epub 2022 Feb 22.
A bovine hemoglobin (HbBv) or human adult hemoglobin (HbA) wrapped covalently by human serum albumins (HSAs), hemoglobin-albumin clusters (HbBv-HSA and HbA-HSA ), are artificial O carriers used as a red blood cell substitute. This article describes the physicochemical properties of the HbBv-HSA and HbA-HSA solutions, and their abilities to restore the systemic condition after resuscitation from hemorrhagic shock in anesthetized rats. The HbBv-HSA and HbA-HSA , which have high colloid osmotic activity, showed equivalent solution characteristics and O binding parameters. Shock was induced by 50% blood withdrawal. Rats exhibited hypotension and significant metabolic acidosis. After 15 min, the rats were administered shed autologous blood (SAB), HbBv-HSA , HbA-HSA , or Ringer's lactate (RL) solution. Survival rates, circulation parameters, hematological parameters, and blood gas parameters were monitored during the hemorrhagic shock and for 6 h after administration. All rats in the SAB, HbBv-HSA , and HbA-HSA groups survived for 6 h. The HbBv-HSA and HbA-HSA groups restored mean arterial pressure after the resuscitation. No remarkable difference was observed in the time courses of blood gas parameters in any resuscitated group except for the RL group. Serum biochemical tests showed increases in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the HbBv-HSA and HbA-HSA groups compared to the SAB group. Therefore, we observed other rats awakened after resuscitation with HbA-HSA for 7 days. The blood cell count, AST, and ALT recovered to the baseline values by 7 days. All the results implied that HbBv-HSA and HbA-HSA clusters provide restoration from hemorrhagic shock as an alternative material for SAB transfusion.
一种由人血清白蛋白（HSA）共价包裹的牛血红蛋白（HbBv）或成人血红蛋白（HbA），即血红蛋白 - 白蛋白聚集体（HbBv - HSA和HbA - HSA），是用作红细胞替代物的人工氧载体。本文描述了HbBv - HSA和HbA - HSA溶液的物理化学性质，以及它们在麻醉大鼠失血性休克复苏后恢复全身状况的能力。具有高胶体渗透活性的HbBv - HSA和HbA - HSA表现出等效的溶液特性和氧结合参数。通过抽取50%的血液诱导休克。大鼠出现低血压和明显的代谢性酸中毒。15分钟后，给大鼠输注自体失血（SAB）、HbBv - HSA、HbA - HSA或乳酸林格氏液（RL）。在失血性休克期间及给药后6小时监测存活率、循环参数、血液学参数和血气参数。SAB、HbBv - HSA和HbA - HSA组的所有大鼠均存活6小时。HbBv - HSA和HbA - HSA组在复苏后恢复了平均动脉压。除RL组外，任何复苏组的血气参数随时间变化均未观察到显著差异。血清生化检测显示，与SAB组相比，HbBv - HSA和HbA - HSA组的天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）升高。因此，我们观察了其他用HbA - HSA复苏7天的大鼠苏醒后的情况。血细胞计数、AST和ALT在7天时恢复到基线值。所有结果表明，HbBv - HSA和HbA - HSA聚集体作为SAB输血的替代材料可使失血性休克得到恢复。
